Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6705-6708.doi: 10.3969/j.issn.1673-8225.2011.36.013

Previous Articles     Next Articles

5-azacytidine induces the differentiation of human umbilical cord derived mesenchymal stem cells into cardiomyocytes

Ruan Zhong-bao1, Yang Xiang-jun1, Chen Ge-cai2, Zhu Li2, Li Wei3, Yang Bing3, Ouyang Xi3, Pan Shao-hui3   

  1. 1Department of Cardiology, the First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China
    2Department of Cardiology, Taizhou People’s Hospital, Taizhou  225300, Jiangsu Province, China
    3Jiangsu BeiKe Bio-Technology, Taizhou 225300, Jiangsu Province, China
  • Received:2011-06-28 Revised:2011-07-13 Online:2011-09-03 Published:2011-09-03
  • Contact: Yang Xiang-jun, Chief physician, Professor, Doctoral supervisor, Department of Cardiology, the First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China yxjdemail@163.net
  • About author:Ruan Zhong-bao☆, Studying for doctorate, Associate chief physician, Department of Cardiology, the First Affiliated Hospital, Soochow University, Suzhou 215006, Jiangsu Province, China ruanzhongbao@medmail.com.cn

PDF

302

Abstract:

BACKGROUND: 5-azacytidine (5-aza) can induce the differentiation of human umbilical cord derived mesenchymal stem cells (hUCMSCs) into cardiomyocytes.
OBJECTIVE: To investigate the cellular mechanism underlying the differentiation of hUCMSCs into cardiomyocytes induces by 5-aza.
METHODS: hUCMSCs were isolated and purified by adherent method. hUCMSCs at passage 3 were induced to differentiate into cardiomyocytes by 5-aza.
RESULTS AND CONCLUSION: Spindle-shaped hUCMSCs were presented from human umbilical cord tissue by adherent culture method. hUCMSCs treated with 5-aza had long cytoplasmic process 1-2 weeks after induction, and they were connected with adjacent cells forming myotube-like structures 3-4 weeks after induction. hUCMSCs treated with 5-aza were stained positively for cTnI 4 weeks after induction. Statistically higher level expression of Nkx2.5 and GATA 4 mRNA in induced group was observed in comparison with those of control group (P < 0.05). These indicated that hUCMSCs can be induced to differentiate into cardiomyocytes by 5-aza in vitro through up-regulating the expression of Nkx2.5 and GATA4.

CLC Number: