Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (36): 6651-6656.doi: 10.3969/j.issn.1673-8225.2011.36.001

    Next Articles

CXCL12/CXCR4 biology axis effects on the repair of spinal cord injury with bone marrow mesenchymal stem cells

Fan Dong-yan1, Liu Yan2, Xu Fu-chun1, Wang Ping3   

  1. 1Tibet University Medical School, Lhasa  850000, Tibet Autonomous Region, China
    2Center Blood Bank of Changchun, Changchun  130033, Jilin Province, China
    3Department of Otolaryngology-Head and Neck Surgery, First Hospital of Jilin University, Changchun  130021, Jilin Province, China
  • Received:2011-04-26 Revised:2011-06-13 Online:2011-09-03 Published:2011-09-03
  • Contact: Wang Ping, Doctor, Department of Otolaryngology-Head and Neck Surgery, First Hospital of Jilin University, Changchun 130021, Jilin Province, China wangping99@ hotmail.com
  • About author:Fan Dong-yan☆, Doctor, Tibet University Medical School, Lhasa 850000, Tibet Autonomous Region, China Dongyan_fan@ yahoo.com.cn

PDF

649

Abstract:

BACKGROUND: The research discovered that bone marrow mesenchymal stem cells (BMSCs) through different approaches can migrate to the injured site of the spinal cord, and play curative effects.
OBJECTIVE: To discuss the role of CXCL12/CXCR4 biology axis on the migration of BMSCs into the injured site after spinal cord injury (SCI).
METHODS: SCI models were prepared using modified spinal damage method. There was no intervention in the sham operation group beside skin open. In the model group, lumbar intrathecal injection of 5 μL normal saline was used at 2 days after modeling; in the transplantation group, lumbar intrathecal injection of 5μL BMSCs was administrated at 2 days after modeling.
RESULTS AND CONCLUSION: Under the fluorescence microscope, a large mass of labeled cells gathered at the injured site, however, only a few of labeled BMSCs could be seen 1 cm distant from the distal injured site. BMSCs expressed the medium level of CXCL12, and CXCR4 also had a low level expression in the BMSCs. Seven days after SCI, partial CXCL12 expression strengthened, mainly in the cortex region of SCI, but there were no massive expressions of CXCL12 1 cm outside the injured site. CXCR4 protein expression did not present with a time-effect manner. CXCR4 transcriptional level in the transplantation group was obviously higher than that in the sham operation group and model group. At 14 days after SCI, CXCL12 transcriptional level reached the peak, and lowered at 21 days. The local CXCL12 transcriptional level was remarkably higher than that at the distal end. CXCR4 also expressed at the injured site in a time-independent manner. The partial CXCR4 transcriptional level was slightly higher than that at the distal end, but there was no significant difference. The results indicated that the CXCL12/CXCR4 biology axis participates in the migration of BMSCs into the injured zone after SCI.

CLC Number: