Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6135-6138.doi: 10.3969/j.issn.1673-8225.2011.33.014

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Effect of interleukin-1 receptor antagonist on neurofilament protein 200 and glial fibrillary acidic protein expression in a rat model of acute spinal cord injury

Huang Jie-gang1, Zong Shao-hui2, Ou Chao3, Xiong Chun-xiang2, Zhao Yu-xi2   

  1. 1Department of Microbiology, 2Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, 3Central Laboratory of Medical Sciences, Guangxi Medical University Nanning   530021, Guangxi Zhuang Autonomous Region, China
  • Received:2011-04-20 Revised:2011-07-05 Online:2011-08-13 Published:2011-08-13
  • Contact: Zong Shao-hui, Doctor, Associate chief physician, Department of Spine Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China xiaohui3008@126. com
  • About author:Huang Jie-gang★, Master, Lecturer, Department of Microbiology of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China katooo@163.com
  • Supported by:

    Doctoral Fund of Guangxi Medical University, No.308010*; Scienfific Research Fund of Education Department of Guangxi Zhuang Autonomous Region, No.guijiao200710LX063*

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Abstract:

BACKGROUND: Interleukin-1 receptor shows protective effects on spinal cord function recovery in rats with acute spinal cord injury, but the precise mechanism remains poorly understood.
OBJECTIVE: To investigate the effect of interleukin-1 receptor antagonist (IL-1Ra) on neurofilament protein 200 (NF 200) and glial fibrillary acidic protein (GFAP) expression in rat acute spinal cord injury tissue.
METHODS: A total of 90 Sprague-Dawley rats were randomly divided into three groups: sham-operated, normal saline and IL-1Ra treatment group. Acute spinal cord injury models were established by improved Allen’s method. At 1, 48 and 72 hours after model establishment, an 8-mm spinal cord segment was dissected longitudinally from the lesion site in each group. The NF200 and GFAP expression was detected by immunohistochemical staining.
RESULTS AND CONCLUSION: NF200 and GFAP expression in the IL-1Ra trentment group was significantly higher than that in the sham-operated and normal saline group (P < 0.05). These results showed that IL-1Ra can increase NF200 and GFAP expression in the injured spinal cord segment and exhibit protective effect on acute spinal cord injury.

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