Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (33): 6107-6110.doi: 10.3969/j.issn.1673-8225.2011.33.007

Previous Articles     Next Articles

Expression of different neuropeptide receptors on osteoblast surface

Zhang Xue-min1, Ma Wen-hui2, Shi Shu-shan3, Zhang Wei-ping2, Feng Zhi-jun2, Li Ya-fei3, Jia Heng-chuan2, Hu Xing-mao2, Zheng Xiao-hua1   

  1. 1Department of Orthopedics, Hebei National Defence Construction Hospital, Shijiazhuang   050081, Hebei Province, China
    2Department of Orthopedics, Bethune International Peace Hospital, Shijiazhuang   050082, Hebei Province, China
    3Department of Orthopedics, General Hospital of Beijing Military Area Command of Chinese PLA, Beijing   100072, China
  • Received:2011-04-23 Revised:2011-06-30 Online:2011-08-13 Published:2011-08-13
  • About author:Zhang Xue-min, Associate chief physician, Department of Orthopedics, Hebei National Defence Construction Hospital, Shijiazhuang 050081, Hebei Province, China hmq318@hotmail. com

PDF

375

Abstract:

BACKGROUND: A large number of studies have demonstrated that neuropeptide can regulate bone metabolism. There have been five neuropeptides found to participate in the process of bone metabolism.
OBJECTIVE: To observe the expression of different neuropeptides on the surface of osteoblasts of healthy adults.
METHODS: Calcitonin gene-related peptide, tyrosine hydroxylase, P substance, neuropeptide Y monoclonal antibodies were used for immunohistochemical staining to observe the expression of various neuropeptide receptors on the surface of osteoblasts. In addition, semi-quantitative analysis of staining gray scale was performed through the use of computer image analysis system.
RESULTS AND CONCLUSION: Neuropeptide Y, P substance and calcitonin gene-related peptide receptor expression was observed on the surface of osteoblasts of healthy adults and these factors influence cellular biological characteristics after binding with corresponding receptors. The gray value from low to high was in the following sequence, neuropeptide Y, calcitonin gene-related peptide, tyrosine hydroxylase, P substance. Results showed that neuropeptide is an important regulatory factor of biological activity of osteoblasts.

CLC Number: