Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 5918-5922.doi: 10.3969/j.issn.1673-8225.2011.32.007

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Ultrastructural changes following osteogenic differentiation of rat bone marrow mesenchymal stem cells

Pan Sheng-cai1, Tang Yu-jin1, Xie Ke-gong1, Lan Chang-gong1, Lu Xian-zhe1, Li Xiao-feng2   

  1. 1Department of Spinal and Bone Disease Surgery, the Affiliated Hospital, Youjiang Medical University for Nationalities, Baise  533000, Guangxi Zhuang Autonomous Region, China
    2Department of Traumatic Orthopaedics and Hand Surgery, the First Affiliated Hospital, Guangxi Medical University, Nanning  530021, GuangxiZhuang Autonomous Region, China
  • Received:2011-05-17 Revised:2011-06-28 Online:2011-08-06 Published:2011-08-06
  • Contact: Tang Yu-jin, Master, Chief physician, Department of Spinal and Bone Disease Surgery, the Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China tangyujin196709@163.com
  • About author:Pan Sheng-cai★, Master, Attending physician, Department of Spinal and Bone Disease Surgery, the Affiliated Hospital, Youjiang Medical University for Nationalities, Baise 533000, Guangxi Zhuang Autonomous Region, China wwwww365@163.com

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Abstract:

BACKGROUND: There are few studies on ultrastructure characteristics of osteoblasts after differentiation of bone marrow mesenchymal stem cells (BMSCs)
OBJECTIVE: By using the whole bone marrow adherence method, to isolate, culture, purified BMSCs from rats, and to observe the ultrastructural changes after osteogenic differentiation of rat BMSCs.
METHODS: BMSCs from rats were isolated, cultured and purified by the whole bone marrow adherence method; for morphology observations, cell surface markers were assessed by flow cytometry, and BMSCs were induced to differentiate into osteoblasts and stained to identification. Scanning electron microscope and transmission electron microscope were used to observe the ultrastructural changes of cells before and after induction.
RESULTS AND CONCLUSION: The third generation cultured BMSCs had high purity and strong vitality. After induction, BMSCs were positive in bone alkaline phosphatase activity and calcified nodules staining. Scanning electron microscope and transmission electron microscope observation showed that after osteogenic induction, the cells spread, arranged irregularly, and were rich of organelles; and the cell mitochondria, rough endoplasmic reticulum, vacuoles increased significantly, indicating that the cells were functionally active.

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