Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (34): 6278-6282.doi: 10.3969/j.issn.1673-8225.2010.34.003

Previous Articles     Next Articles

Injectable nano-hydroxyapatite/chitosan combined with bone marrow stromal cells for repair of bone defects

Liu Yang1, Zhu Li-xin1, Yang Hong3, Tian Jing1, Xu Yong1, Yu Bo1, Li Zhi-hao1, Feng Qing-ling2, Huang Zhi2   

  1. 1 Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China; 2 Department of Materials and Engineering, Tsinghua University, Beijing 100084, China; 3 People’s Hospital of Hualong in Panyu District, Guangzhou 510600, Guangdong Province, China
  • Online:2010-08-20 Published:2010-08-20
  • Contact: Tian Jing, Associate chief physician, Professor, Master’s supervisor, Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China Jing-tian@msn.com
  • About author:Liu Yang★, Studying for master’s degree, Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China liuy0802@126.com
  • Supported by:

    The Science and Technology Project of Guangdong Province, No 2008B030301347*

PDF

675

Abstract:

BACKGROUND: Injectable nano-hydroxyapatite/chitosan (nano-HA/CS) composite is an ideal tissue engineering new material manufactured by Tsinghua University using bionics principles, previous in vitro experiments have demonstrated their good biocompatibility and bone conduction.
OBJECTIVE: To study the ability of bone marrow stromal cells (BMSCs) combined with injectable nano-HA/CS to repair large bone defects of rabbit femoral condyle.
METHODS: Rabbit BMSCs were isolated and cultured with density gradient centrifugation method and adhesive culture method. The third generation of BMSCs mixed with nHA/CS. Bone defects were produced by drilling holes in the femoral lateral condyle in each of 24 New Zealand white rabbits. All the right femoral lateral condyle defects were filled with the BMSCs-nHA/CS composite, as treatment group, while 20 of the left side bone defects were filled with nHA alone, as control group. The other 4 rabbits of the left side bone defects were untreated, as blank control group. The ability of repairing rabbit bone defects was evaluated by gross observation, X-ray, histopathological at the end of 12 weeks after operation.
RESULTS AND CONCLUSION: In treatment group, the implant achieved bone union with bone defects, with obvious new bone formation, and bone defects were repaired completely. In control group, bone defects were repaired partly, with some nonunion. In the blank control group, no osteogenesis was found in the defect area and the defects were filled with fibrous connective tissue. The new formed bone in the treatment group was confirmed integral and trabecular-like structure was formed after 12 weeks, while a little of new bone formed in the control group, some connective tissues grew into the defect. There were no bone formation cells and many fibrous tissues in blank control group. Results showed that, BMSCs-nHA/CS is effective in repairing bone defects than the nHA.

CLC Number: