Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2125-2128.doi: 10.3969/j.issn.1673-8225.2011.12.009

Previous Articles     Next Articles

Preparation of collagen-heparin sulfate nerve bionic scaffold

Li Xiao-long1, Mu Chang-zheng2, Ma Yun-sheng2   

  1. 1Department of Microscopic Central Laboratory, 2Department of Histology and Embryology, Liaoning Medical University, Jinzhou   121000, Liaoning Province, China
  • Received:2010-09-11 Revised:2010-10-03 Online:2011-03-19 Published:2011-03-19
  • Contact: Ma Yun-sheng, Master, Lecturer, Department of Histology and Embryology, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China yunshengma@126.com
  • About author:Li Xiao-long, Experimentalist, Department of Microscopic Central Laboratory, Liaoning Medical University, Jinzhou 121000, Liaoning Province, China yunshengma@126.com?
  • Supported by:

    a grant by Liaoning Provincial Education Department, No. 2008403*; Innovation Team of Liaoning Provincial Education Department, No. 2006T062*

PDF

361

Abstract:

BACKGROUND: Collagen-amino polysaccharide material matrix implanted into segmental injury site surrounding nerve tissues, can promote axonal repair and regeneration. Heparin sulfate is an important component of extracellular matrix.
OBJECTIVE: To prepare the collagen-heparin sulfate nerve tissue engineered scaffold, and to study the histocompatibility for repairing neural injury.
METHODS: Collagen-heparin sulfate turbid liquid was frozen and dried, the inner pore structure was observed with gross observation and scanning electron microscope, the pore size was also measured. Twelve SD rats were used to prepare 5-mm sciatic nerve defect models, which were then divided into three groups: normal control group (autologous nerve transplantation), blank control group and collagen-heparin sulfate composite scaffold transplantation group. Morphology characteristic was observed after transplantation and the repair effect was determined.
RESULTS AND CONCLUSION: Collagen-heparin sulfate scaffolds had longitudinal micro-tubular structure with parallel arrangement, and three-dimensional structure of bionic nerve. Histological staining and electron microscopy showed regenerated nerve fibers successfully grew into the scaffold, some rats’ motor functions were good. Collagen-heparin sulfate material has well histocompatibility, it may promote neural regeneration and be used for neural injury repair.

CLC Number: