Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (12): 2120-2124.doi: 10.3969/j.issn.1673-8225.2011.12.008

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Ectopic osteogenesis of poly-L-lactide porous scaffolds combined with human umbilical cord mesenchymal stem cells in vivo

Sui Jie1, Li Zhi-zhong1, Sun Guo-dong1, Jiao Gen-long1, Li Li-hua2, Zhou Chang-ren2   

  1. 1Department of Orthopaedics, First Affiliated Hospital of Jinan University, Guangzhou  510632, Guangdong Province, China
    2Department of Materials Science and Engineering, College of Science and Engineering, Jinan University, Guangzhou  510632, Guangdong Province, China
  • Received:2010-12-23 Revised:2011-02-17 Online:2011-03-19 Published:2011-03-19
  • Contact: Li Zhi-zhong, Chief physician, Professor, Department of Orthopaedics, First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China
  • About author:Sui Jie★, Studying for master’s degree, Physician, Department of Orthopaedics, First Affiliated Hospital of Jinan University, Guangzhou 510632, Guangdong Province, China suijie1232003@yahoo.com.cn
  • Supported by:

    the National Natural Science Foundation of China, No. 30900307*; the National High Technology Research and Development Program of China, No. 2007AA091603*

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Abstract:

BACKGROUND: Poly-L-lactide (PLLA) material has good supporting effect and provides place for cell adhesion, proliferation and differentiation as 3-D template action.
OBJECTIVE: To study the feasibility and results of ectopic bone formation of PLLA porous scaffolds combined with human umbilical cord mesenchymal stem cells (hUCMSCs).
METHODS: PLLA porous scaffolds were prepared. The hUCMSCs were isolated from human umbilical cord by collagenase digestion. After serial subcultivation in vitro, the hUCMSCs were passaged and identified. Then induce their osteogenic differentiation, be tested and evaluated by specific staining methods. The PLLA porous scaffolds combined with hUCMSCs were implanted into thighs of rabbits at 7 days after osteogenic induction. The bone formation was observed by histopathologic study at 4 weeks after operation.
RESULTS AND CONCLUSION: hUCMSCs grew well on the surface of the PLLA porous scaffolds. These cells were positive for alkaline phosphate staining after osteogenic induction. The mineralized PLLA porous scaffolds combined with hUCMSCs formed obvious block tissues at 4 weeks after implantation, which was hard and constituted of blood vessels examined by histological examination. It is revealed that PLLA porous scaffolds have not affect hUCMSCs proliferation; PLLA porous scaffolds combined with hUCMSCs exhibit the greatest ectopic osteogenesis potential in vivo.

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