Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 2002-2005.doi: 10.3969/j.issn.1673-8225.2011.11.025

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Growth status of BEL-7402 and HepG-2 cells in RPMI-1640 versus DMEM media

Zhao Xiang, Zhang Sha, Xiao Jun-jun, Lin Ming   

  1. Department of Cell Biology, Peking University Health Science Center, Beijing  100191, China
  • Received:2010-10-29 Revised:2011-01-21 Online:2011-03-12 Published:2011-03-12
  • Contact: Lin Ming, Associate chief technician, Department of Cell Biology, Peking University Health Science Center, Beijing 100191, China linminga@bjmu. edu. cn Correspondence to: Xiao Jun-jun, Associate professor, Department of Cell Biology, Peking University Health Science Center, Beijing 100191, China xiaojunjun@bjmu. edu.cn
  • About author:Zhao Xiang, Technician-in-charge, Department of Cell Biology, Peking University Health Science Center, Beijing 100191, China xiangzh@bjmu. edu.cn
  • Supported by:

    the 985 Key Subject of Cell Biology in Peking University*

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Abstract:

BACKGROUND: The most popular commercialized media are DMEM and RPMI-1640. Yet direct comparative study of the two popular media on the influence of liver cancer cell growth has not been reported.
OBJECTIVE: Compare the effect of two popular culture media on growth and proliferation of human hepatocarcinoma BEL-7402 and HepG2 cells, in order to find a more suitable medium.
METHODS: BEL-7402 and HepG2 cells were cultured in complete growth media prepared with either the high glucose-DMEM or the RPMI-1640 medium. Growth and proliferation rates were detected at timed intervals by the acid phosphatase assay. Cellular morphology was observed under an inverse light microscope.
RESULTS AND CONCLUSION: Compared with the DMEM medium, BEL-7402 and HepG2 cells cultured in the RPMI-1640 medium showed a significantly higher growth and proliferation rates (P < 0.01). The cells also revealed better adhesive and stretching status in the RPMI-1640 medium, as confirmed under a microscope. The RPMI-1640 medium is the first choice for tumor cell cultures.

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