Abstract:

BACKGROUND: Cartilage repair has been an orthopedic therapy problem and a hot research spot. With the development of tissue engineering in recent years, the idea of the application of seed cells induced into cartilage cells and the construction of tissue engineered cartilage repair of cartilage defects has received much attention, which has obtained some achievements.
OBJECTIVE: To investigate the selection of seed cells of tissue engineered cartilage, culture condition of chondrogenesis directional induced differentiation, especially for the effects of cell factors, induction methods, culture condition, and other factors.
METHODS: The articles in CNKI and PubMed database (2000-01/2010-09) were retrieved by computer. The index words were “chondrification, cartilage defect, stimulating  actor, cartilage repair, tissue engineering” in Chinese and in English, respectively. Literatures were limited to Chinese and English languages. The foundation and clinical research of chondrification, induced differentiation, tissue engineering scaffolds were included, outdated and repetitive researches were excluded. Totally 155 literature were obtained from the computer screen, according to the inclusion and exclusion criteria, 41 documents of them were included finally.
RESULTS AND CONCLUSION: Seed cells under the effect of certain cell factors can be directed to differentiate into cartilage cells. It can provide new ideas for successful treatment of articular cartilage defect by selecting appropriate seed cells and cell factors induced into cartilage tissue engineering. Directional cartilage differentiation factor transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), osteogenin, insulin-like growth factor (IGF), growth hormone (GH) were control induced by medium additives, gene transfer, different training methods, and other ways to promote the transformation into cartilage. With the deep investigation addressing induced differentiation of cartilage, this research provides effective basis for the problem of articular cartilage defect repair solved by cartilage tissue engineering in clinical application, and has a wide prospect of clinical application.