Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 7004-7007.doi: 10.3969/j.issn.1673-8225.2010.37.039

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Delivery of small-interfering RNA to the mammalian brain

Zhang Qi, Zhang Lin, Yan Rong, Yang Xin-yu   

  1. Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin Neurological Institute, Tianjin  300052, China
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Yang Xin-yu, Doctor, Professor, Chief physician, Master’s supervisor, Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin Neurological Institute, Tianjin 300052, China paperxyyang@gmail.com
  • About author:Zhang Qi★, Studying for master’s degree, Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin Neurological Institute, Tianjin 300052, China Dake413@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30973090*; Natural Science Foundation of Tianjin, No. 09JCZDJC17200*; High School Science & Technology Fund Planning Project of Tianjin, No. 20070202*

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Abstract:

BACKGROUND: As a new method of gene therapy, small-interfering RNA (RNAi) can reduce the expression of target gene, which has a great potential in the treatment of neuroscience diseases. By introducing siRNA with the same sequence of endogenous target gene, the technology can artificially induce sequence-specific mRNA degradation to prevent the gene expression.
OBJECTIVE: To explore the delivery of siRNA to the mammalian brain in the treatment of neuroscience diseases.
METHODS: The database of PubMed home was retrieved with key words of “siRNA, delivery, brain” and “siRNA, delivery, vivo” by computer, separately. The literature was selected according to inclusion and exclusion criteria. Totally 27 papers were analyzed from the synthesis of siRNA, delivery in the brain, its inhibition efficiency and side effects.
RESULTS AND CONCLUSION: In recent RNAi studies, high-precision prediction method of siRNA could be used to synthesize highly specific siRNA; siRNA might be delivered to the mammalian brain, mainly through local injection approach, and with the help of the delivery carrier, the inhibition efficiency was constantly improved; with the study of RNAi technology, its side effects was also gaining the attention of researchers. In brief, as a novel treatment strategy, RNAi has a great potential in the treatment of neuroscience diseases and can bring a new revolution in drug discovery and development.

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