Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1823-1826.doi: 10.3969/j.issn.1673-8225.2011.10.024

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Biological characteristics of human chorionic-derived mesenchymal stem cells

Zhang Rui-ting1, Han Zhi-bo2, 3, Wang Tao4, Meng Lei2, 3, Liu Zhi-peng1, Li Kui4, Ji Yue-ru3, Yang Zhou-xin3, Li Yang-qiu1, Han Zhong-chao1, 2, 3   

  1. 1School of Medicine, Jinan University, Guangzhou  510632, Guangdong Province, China
    2TEDA Life Science and Technology Research Center, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin  300457, China
    3State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin  300020, China
    4Guangzhou Health & Biotech Co., Ltd., Guangzhou  510632, Guangdong Province, China
  • Received:2010-11-02 Revised:2010-12-06 Online:2011-03-05 Published:2011-03-05
  • Contact: Han Zhong-chao, Doctor, Doctoral supervisor, School of Medicine, Jinan University, Guangzhou 510632, Guangdong Province, China; TEDA Life Science and Technology Research Center, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300457, China; State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300020, China hanzhongchao@hotmail.com
  • About author:Zhang Rui-ting★, Studying for master’s degree, School of Medicine, Jinan University, Guangzhou 510632, Guangdong Province, China zhangruiting66@foxmail.com
  • Supported by:

    Guangdong Science and Technology Department, the Cooperation Project in Industry, Education and Research of Guangdong Province and Ministry of Education of P.R. China (The Industrialization Base of Stem Cell of Guangdong Province, No. 2010B091101004) *

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Abstract:

BACKGROUND: Bone marrow is major source of mesenchymal stem cells (MSCs), but the quantity and quality of MSCs in bone marrow have been shown to be gradually reduced with the increase of age. Therefore, it’s very important to find a new source of stem cells.
OBJECTIVE: To study a new source of MSCs isolated from human chorionic villous and to study its biological characteristics.
METHODS: Umbilical cord and amniotic membrane were isolated from clean placenta; the remaining placenta tissue underwent primary culture and subculture. Tandem repetitive sequence (TRS) analysis was used to detect whether the MSCs obtained from placenta is chorionic villous original. Cell growth pattern was detected by MTT assay. Flow cytometry was applied to analyze cell phenotype and stem cell markers. The different differential medium was used to detect its multi-directional differentiation ability.
The immunomodulatory properties of these chorionic-derived cells were evaluated by coculturing the cells with peripheral blood mononuclear cells stimulated by phytohemagglutinin (PHA), and the level of interferon-γ (IFN-γ) was detected by enzyme-labeled immunosorbent assay (ELISA).
RESULTS AND CONCLUSION: TRS analysis shows that these chorionic-derived cells are derived from human chorionic. They are adherent cells with typical fibroblast morphology. Flow cytometric analysis reveals that human chorionic-derived cells are consistently positive for MSCs labeled with CD73, CD29, CD44, CD90 and CD105, and negative for the hematopoietic markers CD45, CD11b and CD34. The cells can differentiate into osteoblasts and adipogenesis under different conditioned medium cultured conditions. These results proved that these cells are human chorionic-derived MSCs. The human chorionic-derived MSCs can inhibit peripheral blood mononuclear cell stimulated by PHA to secrete γ-interferon.

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