Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1814-1818.doi: 10.3969/j.issn.1673-8225.2011.10.022

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Expressions of nuclear factor κBp65 and proliferating cell nuclear antigen in injured carotid artery balloon after bone marrow mesenchymal stem cells transplantation

Liu Zhi-jiang, Shi Bei, Xu Guan-xue, Zhao Ran-zun, Shen Chang-yin, Wang Dong-mei, Wang Zheng-long   

  1. Department of Cardiology, First Affiliated Hospital of Zunyi Medical College, Zunyi  563003, Guizhou Province, China
  • Received:2010-10-10 Revised:2010-11-23 Online:2011-03-05 Published:2011-03-05
  • Contact: Shi Bei, Professor, Master’s supervisor, Department of Cardiology, First Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China shibei2147@yahoo.com.cn
  • About author:Liu Zhi-jiang★, Master, Attending physician, Department of Cardiology, First Affiliated Hospital of Zunyi Medical College, Zunyi 563003, Guizhou Province, China liuzhijiang348@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. NSFC30860100*

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Abstract:

BACKGROUND: Inflammatory reaction plays an important role in the incidence of restenosis after percutaneous transluminal coronary intervention. Recent studies found that bone marrow mesenchymal stem cells (BMSCs) have strongly characteristics of immunological regulation. However, BMSCs on the inflammatory reaction in vascular injury is rarely reported.
OBJECTIVE: To investigate effects of BMSCs transplantation on expression of nuclear factor κBp65 (NF-κBp65) and proliferating cell nuclear antigen (PCNA) after carotid artery balloon injury of rabbits, and to analyze its correlation with intimal proliferation.
METHODS: Thirty-six rabbits were prepared for carotid artery atherosclerotic stenosis models and were randomly divided into the control group and BMSCs transplantation group. BMSCs pre-labled by DAPI was infused into rabbits by the ear vein in the BMSCs transplantation group, and the same amount of PBS solution was infused in the control group. The plasma tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were detected by ELIAS in prior to model preparation and at 7, 14, 28 days after transplantation. The NF-κBp65 and PCNA expressions in vessels tissues were detected by immunohistochemical analysis at 14 days after transplantation, and vascular morphological changes were determined by hematoxylin-eosin staining at 28 days after transplantation.
RESULTS AND CONCLUSION: The expression of NF-κBp65 and PCNA in the BMSCs transplantation group decreased significantly compared with control group at 14 days after transplantation (P < 0.05). At 7, 14, and 28 days after transplantation, the plasma TNF-α and IL-6 levels in the BMSCs transplantation group were significantly lower than those in the control group (P  < 0.05). The intimal area, the ratio of the intima/media area and the luminal stenosis ratio were significantly decreased in the BMSCs transplantation group than control group at 28 days after transplantation (P  < 0.05). BMSCs is capable to decrease the inflammatory reaction of injured vessels and down-regulate PCNA expression by inhibiting NF-κBp65 in injured vessels, and BMSCs may be good for inhibition of injured vessels intimal proliferation.

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