Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1794-1798.doi: 10.3969/j.issn.1673-8225.2011.10.018

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Effects of long term intranasal delivering umbilical cord blood mononuclear cells on cerebral infarct volume and behaviors in rats

Zeng Xian-zhi1, Shen Hui-juan2, Wang Yang3, Zhu Qun-e4, Guo Lian-jun4, Ye Xian-cai4   

  1. 1Department of Anatomy, College of Medicine, Jiaxing University, Jiaxing  314001, Zhejiang Province, China; 2Laboratory Center of  Machinery and Electronics Engineering, College of Machinery and Electronics Engineering, Jiaxing University, Jiaxing  314001, Zhejiang Province, China; 3Clinical Department, College of Medicine, Jiaxing University, Jiaxing  314001, Zhejiang Province, China; 4Medical Laboratory Center, College of Medicine, Jiaxing University, Jiaxing  314001, Zhejiang Province, China
  • Received:2010-12-24 Revised:2011-02-07 Online:2011-03-05 Published:2011-03-05
  • About author:Zeng Xian-zhi☆, Doctor, Lecturer, Department of Anatomy, College of Medicine, Jiaxing University, Jiaxing 314001, Zhejiang Province, China frankzengxzh@yahoo.cn
  • Supported by:

    the Science and Technology Program of Jiaxing City of Zhejiang Province, No. 2010AY1097*; the Science and Technology Innovation Program of Undergraduate of Zhejiang Province, No. 2010R417021*

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Abstract:

BACKGROUND: It was a new non-invasive method for intranasal delivering cell or cytokine into the brain, which could overcome the peripheral side-effect of other delivering methods.
OBJECTIVE: To explore the effect of long term intranasal delivering human umbilical cord blood mononuclear cells (UCBMCs) on changes in behaviors and infarct volume of brain infarct in rats.
METHODS: Middle cerebral artery of Wistar rat was directly cut to establish models of cerebral infarction. Rat models with equal neurological score were randomly assigned to control and mononuclear cell groups. In the mononuclear cell group, UCBMCs of the third generation were intranasally injected. In the control group, phosphate buffer saline was infused, once a day, totally 6 weeks. Behavior was assessed once a week. Brain infarct volumes were quantified following the last behavior assessment.
RESULTS AND CONCLUSION: Compared with control group, modified neurological severity score was improved in the mononuclear cell group at 3 weeks after treatment, and lasted at 5 weeks (P < 0.05, P < 0.01). In the mononuclear cell group, the mean latency in Morris Water Maze Test shortened significantly at 1-5 weeks following treatment (P < 0.05, P < 0.01), and the effect was obvious over time. Identical to behavior improvement, the cerebral infarct volume in the mononuclear cell group (9.15±4.36)% was smaller compared with (30.56±4.65)% (P < 0.01). Early and long term of intranasal delivering UCBMCs to treat brain infarct model rats could improve their behavior score and shrink their infarct volume, which could be considered as a simple and feasible method to treat brain infarct.

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