Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1726-1730.doi: 10.3969/j.issn.1673-8225.2011.10.004

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Effects of myocardium lysate on the differentiation of rat bone marrow-derived mesenchymal stem cells into cardiac myocyte in vitro

Li Qin1, Zhao Wen-jing1, Kou Ya-li1, Hu Li-xia2, Chen Wei-ping1   

  1. 1Department of Histology and Embryology, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
    2Department of Obstetrics, Xinxiang Central Hospital, Xinxiang  453000, Henan Province, China
  • Received:2010-11-24 Revised:2011-01-11 Online:2011-03-05 Published:2011-03-05
  • Contact: Chen Wei-ping, Professor, Master’s supervisor, Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China chwp8852@126.com
  • About author:Li Qin★, Master, Department of Histology and Embryology, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China lq740036@163.com
  • Supported by:

    the Item of Science and Technology Department of Guangxi, No. Guikeqing0229009*; the Item of Science and Technology Department of Guangxi, No. Guikegong0592007-1G*; the Item of Science and Technology Department of Guangxi, No. Guikezi0832157*

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Abstract:

BACKGROUND: Stem cells have the characteristics of “differentiation dependent on enviroment”, little research have focused on myocardium lysate that simulating the microenvironment of cardiocyte induce bone marrow mesenchymal stem cells (BMMSCs) differentiated into cardiocyte-like cells.
OBJECTIVE: To investigate the effects of myocardium lysate on the differentiaion of bone marrow mesenchymal stem cells into myocardial-like cell.
METHODS: Changes of morphological characteristics of the second passaged BMMSCs that cultured with medium contained myocardium lysate for 5 weeks were observed. After the procedure of induction, α-Actin was used to observe whether BMMSCs differentiate into cardiocyte-like cells, hematoxylin-eosin staining was used to identify whether intercalated disk was existed, while electron microscopys was used to assess the ultrstructure of the induced cells.
RESULTS AND CONCLUSION: Most of the induced cells like bamboo in microscopys, positive reaction after α-actin immunocytochemical stain showed that the BMMSCs were differentiated into myo-like cells. Intercalated disk, well sequenced sarcomeres and myofilaments of the cytoplasm observed revealed the bamboo-like cells have the typical morphology of cardiocyte. Spontaneous beating myotube-like structures generated at 12 days after induction. In brief, the myocardium lysate can imitating the microenvironment of cardiocyte, induce BMMSCs differentiated into cardiocyte-like cell which have the typical morphology of cardiocyte and the corresponding functional activities.

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