Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1715-1720.doi: 10.3969/j.issn.1673-8225.2011.10.002

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Effects of high-frequency pulsed electromagnetic fields on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Cui Xiang-rong, Su Wei, Huang Zhao, Qin Wan-an   

  1. Hand Surgery, Department of Trauma Osteology, First Affiliated Hospital, Guangxi Medical University, Nanning  530021, Guangxi Zhuang Autonomous Region, China
  • Received:2010-10-24 Revised:2010-11-26 Online:2011-03-05 Published:2011-03-05
  • Contact: Su Wei, Professor, Chief physician, Hand Surgery, Department of Trauma Osteology, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China gxsuwei@163.com
  • About author:Cui Xiang-rong★, Studying for master’s degree, Hand Surgery, Department of Trauma Osteology, First Affiliated Hospital, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China crrrr@163.com
  • Supported by:

    the National Natural Science Foundation of China, No.30860078*; the Science and Research Program from Education Department of Guangxi Zhuang Autonomous Region, No. 200710MS021*; the Science and Research Fund from the First Affiliated Hospital of Guangxi Medical University*

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Abstract:

BACKGROUND: The researches about low-frequency pulsed electromagnetic fields (PEMFs) interfering with bone marrow mesenchymal stem cells (BMSCs) proliferation and differentiation are many, but there is no report concerning high-frequency (> 300 MHz) PEMFs.
OBJECTIVE: To explore whether high-frequency (> 300 MHz) PEMFs can promote proliferation and osteogenic differentiation of BMSCs.
METHODS: BMSCs were isolated from Sprague-Dawley rats. The third passage of BMSCs were obtained and divided into four groups: osteogenic induction group, osteogenic induction+PEMF irradiation group, PEMF irradiation group and blank control group. Changes in morphology, number and amount of total protein were observed during BMSC culture in each group.
RESULTS AND CONCLUSION: The number of BMSC bodies was increased, but the differentiation ability was weak following PEMF irradiation. The speed of cell proliferation and content of total protein were lower in the PEMF irradiation group compared with blank control group (P < 0.05). However, cell apoptotic rate was greater in the PEMF irradiation group compared with blank control group (P < 0.05). Results indicated that promoting effects of high-frequency PEMFs on osteogenic differentiation of BMSCs are not obvious. High-frequency PEMFs can suppress BMSC proliferation and contribute to BMSC apoptosis.

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