Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (37): 6917-6921.doi: 10.3969/j.issn.1673-8225.2010.37.018

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Establishment of a Alzheimer’s disease rat model intervened by beta-amyloid protein 1-40 and aluminum chloride

Liu Zhi-an 1, 2, Zhao Wei-wei3, Ma Kai4, Lü Chun-e2, Xu Tie-jun 1, 2   

  1. 1 Jiangsu Province Key Laboratory of Brain Disease Bioinformation, 2 Department of Anatomy and Neurobiology, 4 Department of Computer, Xuzhou Medical College, Xuzhou  221002, Jiangsu Province, China; 3 Department of Radiology, Affiliated Xijing Hospital of Fourth Military Medical University, Xi’an  710032, Shaanxi Province, China　
  • Online:2010-09-10 Published:2010-09-10
  • Contact: Xu Tie-jun, Doctor, Professor, Doctoral supervisor, Jiangsu Province Key Laboratory of Brain Disease Bioinformation, Department of Anatomy and Neurobiology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China xztjxu@163.com
  • About author:Liu Zhi-an☆, Studying for doctorate, Associate professor, Master’s supervisor, Jiangsu Province Key Laboratory of Brain Disease Bioinformation, Department of Anatomy and Neurobiology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China zhianliu@126.com
  • Supported by:

     the Open Research Fund Program of the Jiangsu Key Laboratory of Brain Disease Bioinformation, No. JSBL0903*; Program for Postgraduates Research Innovattion in University of Jiangsu Province, No. [2009]10*; Natural Science Foundation of Colleges and Universities in Jiangsu Province, No. 07KJB310117*

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Abstract:

BACKGROUND: The existed Alzheimer's disease (AD) animal models have certain limitations.
OBJECTIVE: To establish an ideal and practical AD rat model intervened by β-amyloid protein-40 (Aβ1-40) and AlCl3.
METHODS: Healthy male aged Sprague Dawley rats were randomly divided into AD model group, saline group and normal control group after Morris water maze training and screening. Rat AD models were established by one-time unilateral intracerebroventricular injection of Aβ1-40 and continuous intraperitoneal injection of AlCl3 every other day for 4 weeks. The behavioral changes of model rats were detected by Morris water maze test and step-down test. The hippocampal amyloid deposition and pathology changes of rats were determined by Congo red and hematoxylin-eosin staining.
RESULTS AND CONCLUSIONS: Compared with the normal control group and saline group, the water maze test escape latency of model group rats was significantly longer (P < 0.05). The reaction time, basal number of errors, and number of errors of step-down test were increased significantly (P < 0.05). The latency period of step-down test was shortened significantly (P < 0.05). The amyloid substance deposition, as well as the obvious damage changes in morphology and reduction of cells number could be seen in hippocampus of model group rats. These results suggest that an ideal and practical AD rat model can be successfully established with Aβ1-40 and AlCl3.

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