Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (40): 7447-7452.doi: 10.3969/j.issn.1673-8225.2010.40.009

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Dose-depended effects of bovine pituitary extract, basic fibroblast growth factor and Forskolin on olfactory ensheathing cell proliferation

Yang Bo-yu1, Wang Feng2, Wang Wei3   

  1. 1 Jimei Department of Orthopaedics, Xiamen Second Hospital, Xiamen  361021, Fujian Province, China; 2 Department of Human Anatomy, Histology and Embryology, Fujian Medical University, Fuzhou  350001, Fujian Province, China; 3 Neurobiology Research Center, Fujian Medical University, Fuzhou  350001, Fujian Province, China
  • Online:2010-10-01 Published:2010-10-01
  • About author:Yang Bo-yu★, Master, Attending physician, Jimei Department of Orthopaedics, Xiamen Second Hospital, Xiamen 361021, Fujian Province, China hly76718@sina.com

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Abstract:

BACKGROUND: Growth factors participate in regulating proliferation, differentiation and metabolism of olfactory ensheathing cells (OECs). Therefore, reasonable sequential stimulation form can be used to reach multiple amplification of cell number.
OBJECTIVE: To investigate the effect of basic fibroblast growth factor (bFGF), bovine pituitary extract and Forskolin on OECs proliferation as well as dose-effect relationship.
METHODS: The OECs were isolated from the neonatal rat olfactory bulb using primary culture technique. The OECs were purified using differential velocity adherent + chemicals + trypsin digestion method. The bFGF, bovine pituitary extract and Forskolin alone and their combination were added. MTT assay was utilized to detect OEC growth in each group. The purity of the OECs was evaluated and growth rate of OECs was calculated using NGFR p75 immunocytochemistry.
RESULTS AND CONCLUSION: Following primary culture and purification of OECs, bovine pituitary extract could facilitate OECs growth, but typical dose-effect relationship was not found. bFGF could facilitate OECs growth, which presented typical dose-effect relationship. An additional Forskolin could not promote OEC proliferation. These indicated that combined use of bFGF and Forskolin in a proper concentration significantly enhanced cell proliferation. Results suggest that sequent use of combined growth factors in a proper concentration would be a reasonable strategy to effectively enhance cell proliferation and this might be an ideal way in dealing with seed cell scarcity for treatment with spinal cord injury.

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