Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (53): 9994-9998.doi: 10.3969/j.issn.2095-4344.2012.53.022

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Identification of a novel human leukocyte antigen-DPA1*01:11 allele in a Chinese Han population

He Liu-mei, Wang Da-ming, Xu Yun-ping, Deng Zhi-hui   

  1. Shenzhen Blood Center, Shenzhen 518020, Guangdong Province, China
  • Received:2012-04-19 Revised:2012-08-26 Online:2012-12-30 Published:2012-12-30
  • Contact: Deng Zhi-hui, Doctor, Chief technician, Shenzhen Blood Center, Shenzhen 518020, Guangdong Province, China zhihui_deng@yahoo.com.cn
  • About author:He Liu-mei★, Master, Assistant researcher, Shenzhen Blood Center, Shenzhen 518020, Guangdong Province, China littleduckmei@163.com

Abstract:

BACKGROUND: In population genetics research, an abnormal loci result of human leukocyte antigen-DPA1 sample was found, while the international reports on the human leukocyte antigen-DPA1 allele are rare.
OBJECTIVE: To identify a novel human leukocyte antigen-DPA1*01 variant allele in a Chinese Han individual.
METHODS: Sequencing based typing at exon 2 of human leukocyte antigen-APA1 gene in both directions was used in our population genetics study. Samples with in-conclusive results were subjected to molecular cloning and haplotype sequencing.
RESULTS AND CONCLUSION: An abnormal loci result of human leukocyte antigen-DPA1 sample was found, and a sample with in-conclusive result in sequencing based typing test subjected to cloning and haplotype sequencing, one normal HLA-DPA1*01:03 allele and a novel DPA1*01 variant allele were identified. The novel DPA1*01 variant allele differs from the closest allele DPA1*01:03 by single nucleotide change at coding sequence nt242 A>G in exon 2, and leading to the corresponding 50 codons CAA> CGA, thus resulted in encoded amino acid residues changed from glutamine into arginine. The identified novel DPA1*01 variant allele has been officially designated as human leukocyte antigen-DPA1*01:11 by World Health Organization Nomenclature Committee for Factors of Human Leukocyte Antigen System (Submit serial number: HWS10015443).

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