Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (5): 778-782.doi: 10.3969/j.issn.1673-8225.2011.05.005

Previous Articles     Next Articles

Establishment and evaluation of an acute rejection model of orthotopic liver transplantation in inbred rats

Xie Jin-min1,2, Dong Rui-qiang3, Li Tao1, Zhang Hui-ying2, Yao Kun-hou2, Wen Hao1   

  1. 1The Post-doctoral Research Station, First Affiliated Hospital, Xinjiang Medical University, Urumqi  830011, Xinjiang Uighur Autonomous Region, China
    2Department of General Surgery, the 474 Hospital of  Chinese PLA, Urumqi  830011, Xinjiang Uighur Autonomous Region, China
    3Shihezi Medical University, Shihezi  834407, Xinjiang Uighur Autonomous Region, China
  • Received:2010-08-15 Revised:2010-11-06 Online:2011-01-29 Published:2011-01-29
  • Contact: Wen Hao, Doctor, Professor, the Post-doctoral Research Station, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China Dr.wenhao@163.com
  • About author:Xie Jin-min☆, Doctor, Master’s supervisor, Associate chief physician, the Post-doctoral Research Station, First Affiliated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; Department of General Surgery, the 474 Hospital of Chinese PLA, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
  • Supported by:

    the Science and Technology Plan of Guangdong Province, No. 37055*

PDF

464

Abstract:

BACKGROUND: The models commonly used from closed population at present were not well ideal, which easy lead to liver transplantation tolerance.
OBJECTIVE: To establish a stable rat model of acute rejection following allograft liver transplantation from DA to Lewis by two-cuff method.
METHODS: The rats were allocated to the isogene group (n=24, liver grafts from Lewis rat to Lewis rat) or allogene group (n=24, liver grafts from DA rat to Lewis rat). The general states and survival duration of the rats after transplantation were observed. Three recipient rats in each group were randomly executed at 3, 5, 7 and 10 days for observation of histopathological changes in the liver tissues and detection of serum levels of aspartate aminotransferase (AST), total bilirubin, and cytokine.
RESULTS AND CONCLUSION: In the isogene group,a mild morphological change in the liver tissue was found but no acute rejection occurred, which the median survival duration was more than 100 days. In the allogene group, however, obvious jaundice developed postoperatively, which the median survival duration was 11 days, and a typical acute rejection occurred pathologically in the liver tissue at 7 days according to the Banff standards. Levels of AST, total bilirubin, and cytokine were respectively significantly higher in allogene group than that in isogene group at 3, 5, 7 and 10 days (P < 0.001). The acute rejection of DA to Lewis orthotopic liver transplantation model is stable; it can be effectively used to study allograft rejection and tolerance.

CLC Number: