Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (5): 769-773.doi: 10.3969/j.issn.1673-8225.2011.05.003

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Nuclear factor kappa B participates in hypoxia preconditioning influencing JNK pathway in rat orthotopic liver transplantation 

Meng Zhong-liang1, Zhang Pei-jian1, Wu Xue-yan1, Zhuang Zhuo-nan1, Zhang Jie1, Su Hui1, Jiang Yong-jun1, Jin Cheng2   

  1. 1Institute of General Surgical Research, Second Medical College of Yangzhou University, Yangzhou 225000, Jiangsu Province, China
    2Center of Liver, Gall and Pancreas, Wuxi Third Hospital, Wuxi  214000, Jiangsu Province, China
  • Received:2010-08-23 Revised:2010-11-13 Online:2011-01-29 Published:2011-01-29
  • Contact: Zhang Pei-jian, Master, Professor, Chief physician, Master’s supervisor, Institute of General Surgical Research, Second Medical College of Yangzhou University, Yangzhou 225000, Jiangsu Province, China yzu.edu.pjz@163.com
  • About author:Meng Zhong-liang★, Studying for master’s degree, Institute of General Surgical Research, Second Medical College of Yangzhou University, Yangzhou 225000, Jiangsu Province, China mzl913@163.com
  • Supported by:

    the Science and Technology Subjects of Healthy Department of Jiangsu Province, No. H200770*; the Tackle Key Program in Science and Technology of Jiangsu Province, No. YZ2008042*; the Science and Technology Plan for Society Development of Wuxi City*

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Abstract:

BACKGROUND: Nuclear factor κB (NF-κB) and JNK cascade pathway determine the fate of cells in liver ischemia/reperfusion injury. However, there are few reports addressing hypoxia preconditioning-reduces apoptosis during liver transplantation. 
OBJECTIVE: To assess the role and protective effects of NF-κB on JNK pathway mediated by hypoxia preconditioning.
METHODS: An orthopotic liver transplantation model was constructed by portal vein perfusion. SD rats were randomly divided into the following three groups: normal control group: received no treatment; autotransplantation group: performed autotransplantation; and hypoxia preconditioning group: rats were given an 8% oxygen mixed gas for 90 minutes before autotransplantation. At 1, 6 and 24 hours after operation, rats were sacrificed for detection of p-JNK protein, malondialdehyde and superoxide dismutase (SOD) expressions. NF-κB mRNA content was detected by RT-PCR, and the ultrastructure changes of hepatocytes were observed under a transmission electron microscopy. 
RESULTS AND CONCLUSION: Compare to the normal control group, the expression of malondialdehyde level was significant increased, but SOD was obviously decreased (P < 0.05) in the transplantation groups. Compare to the autotransplantation group, the expression of malondialdehyde was decreased significantly, and SOD was increased significantly in the hypoxia preconditioning group (P < 0.05). The expression of p-JNK protein and NF-κB mRNA in the transplantation groups were dramatically increased compared with normal control group (P < 0.05). The NF-κB mRNA level was increased but p-JNK protein expression decreased in the hypoxia preconditioning group compared with autotransplantation group (P< 0.05). Hepatic cells in the autotransplantation group showed typical apoptosis sign under transmission electron microscope, but no apoptosis was found in the hypoxia preconditioning group. The findings demonstrated that hypoxia preconditioning has marked inhibition to apoptosis by upregulating protein expression of NF-κB, preventing reactive oxygen species accumulation and suppressing prolonged JNK activation after orthopotic liver transplantation.

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