Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (26): 5579-5587.doi: 10.12307/2025.770
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Sun Hailiang1, 2, Pang Jian1, 2, 3, Shi Wanzhong1, Shi Ying1, 2, 3
Received:
2024-09-03
Accepted:
2024-10-16
Online:
2025-09-18
Published:
2025-02-25
Contact:
Shi Wanzhong, MD, Chief pharmacist, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Co-corresponding author: Shi Ying, MS, Doctoral supervisor, Chief physician, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shi Traumatology Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Institute of Traumatology & Orthopedics, Shanghai Institute of Traditional Chinese Medicine, Shanghai 201203, China
About author:
Sun Hailiang, Master candidate, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shi Traumatology Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
Supported by:
CLC Number:
Sun Hailiang, Pang Jian, Shi Wanzhong, Shi Ying. Protective effect of Huaizhen Yanggan Capsule on knee osteoarthritis induced by sodium iodoacetate in model mice[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(26): 5579-5587.
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2.2.2 怀珍养肝胶囊对耳郭组织病理改变的影响 空白组小鼠左耳耳郭较为狭窄,血管及肌肉组织分层明显;空白组小鼠右耳耳郭血管及周围纤维组织出现明显的充血、水肿,横纹肌纤维排列紊乱,存在大量炎症细胞浸润;怀珍养肝胶囊干预后,低剂量组仍可见明显的组织水肿,周围血管充血,横纹肌纤维排列紊乱,炎症细胞浸润,但与空白组右耳比较有所好转。随着怀珍养肝胶囊剂量的增加,组织水肿及充血情况得到明显改善,横纹肌纤维排列恢复,炎性细胞浸润减少;塞来昔布组也存在少量充血水肿及炎性细胞浸润,见图1。 2.2.3 怀珍养肝胶囊对耳郭组织中肿瘤坏死因子α、白细胞介素6水平的影响 各组小鼠耳郭组织中肿瘤坏死因子α和白细胞介素6水平,见表2。结果显示,与空白组相比,塞来昔布组和怀珍养肝胶囊各剂量组均可以显著减少耳郭组织中肿瘤坏死因子α、白细胞介素6的分泌,其中怀珍养肝胶囊中、高剂量组与空白组相比在统计学上有显著性差异(P < 0.05)。"
2.4 实验三结果 2.4.1 怀珍养肝胶囊对膝骨关节炎实验小鼠步幅的影响 与空白组相比,模型组小鼠步幅显著减小(P < 0.05);与模型组相比,怀珍养肝胶囊组小鼠步幅显著增大(P=0.018);怀珍养肝胶囊组和空白组小鼠步幅差异不显著(P=0.037),见图2。 2.4.2 怀珍养肝胶囊对膝骨关节炎实验小鼠抗氧化能力的影响 与空白组相比,模型组总抗氧化能力显著下降(P=0.006);与模型组相比,怀珍养肝胶囊组总抗氧化能力显著升高(P=0.010),见图3。 2.4.3 怀珍养肝胶囊对膝骨关节炎实验小鼠感兴趣区松质骨和皮质骨结构及骨体积分数的影响 膝关节Micro-CT 2D和3D重建图像,模型组感兴趣区的皮质骨排列致密,松质骨骨小梁排列错乱无规则,网状结构发生变化,关节腔有游离体,关节内侧有骨赘;与模型组比较,怀珍养肝胶囊组骨破坏得到缓解,骨赘形成得到抑制,见图4。 与空白组比较,模型组感兴趣区的松质骨骨体积分数明显降低(P=0.020);与模型组比较,怀珍养肝胶囊组骨体积分数明显增加(P=0.045),见图5。虽然怀珍养肝胶囊组感兴趣区皮质骨骨体积分数(87.60±0.86)%大于空白组(87.35±1.15)%,模型组(87.73±1.65)%大于怀珍养肝胶"
囊组,但无统计学差异。 2.4.4 怀珍养肝胶囊对膝骨关节炎实验小鼠关节软骨形态的影响 苏木精-伊红和番红O-固绿染色后关节软骨形态,见图6。图中A1-C1为股骨图像,A2-C2为胫骨图像。空白组(图6A1、A2)关节软骨表面光滑,结构完整,层次清晰,软骨细胞排列规则。与空白组相比,模型组(图6B1、B2)小鼠关节软骨存在明显破坏和裂隙,软骨表面不平整,软骨细胞数量明显减少且形态异常,软骨基质染色明显减少,蛋白多糖丢失较多,软骨纤维化增加;与模型组相比,怀珍养肝胶囊组(图6C1、C2)小鼠关节软骨表面较平整,软骨基质染色较好,蛋白多糖丢失较少;与空白组相比,怀珍养肝胶囊组小鼠关节软骨仍存在较明显的软骨细胞和基质破坏。 改良Mankin’s评分:空白组最低,怀珍养肝胶囊组次之,模型组最高,空白组与模型组相比差异有显著性"
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