Biological characteristics of glucose-responsive microcapsules carrying beta-TC3 cells

doi:10.3969/j.issn.2095-4344.2013.29.011

Abstract

Abstract:

BACKGROUND: To prepare glucose-responsive microcapsules which can control insulin release as changing the glucose concentration in the medium is of great significance to control the occurrence and development of diabetes mellitus.
OBJECTIVE: To study the performance of glucose-responsive alginate/modified-chitosan/alginate microcapsules carrying β-TC3 cells.
METHODS: Glucose-responsive alginate/modified-chitosan/alginate microcapsules were prepared by layer-by-layer self-assembly method to evaluate the performance. And the glucose-responsive microcapsules carrying β-TC3 cells were prepared to observe the cell proliferation within the microcapsules.
RESULTS AND CONCLUSION: The integrity rate of glucose-responsive alginate/modified-chitosan/alginate microcapsules could be 95% after 48 hours oscillation, and the hardness of microcapsules lowered, but the elasticity increased. The permeability test showed that microcapsules intercepted macromolecular substances such as bovine serum albumin and immuno-globulin G. The microcapsules could release more insulin with the increase of glucose concentration. As described above, the glucose-responsive alginate/modified-chitosan/ alginate microcapsules had good mechanical strength, immunoisolation effect and glucose sensitivity. The β-TC3 cells entrapped in the glucose-responsive microcapsules could grow well and the peak of cell proliferation lagged behind as compared with non-microencapsulated cells, indicating the glucose-responsive microcapsules had good biocompatibility.

CLC Number:

R318

Zhang Shao-min, Wu Zhong-ming, Yu De-min. Biological characteristics of glucose-responsive microcapsules carrying beta-TC3 cells[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(29): 5331-5336.

Figures/Tables 5

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