Correlation between bone mineral density and serum vascular endothelial growth factor levels in ovariectomized rats

doi:10.3969/j.issn.2095-4344.2013.28.003

Abstract

Abstract:

BACKGROUND: Vascular endothelial growth factor play an important role in promoting healing of osteoporotic fractures, but whether it can affect the bone mineral density is not clear.

OBJECTIVE: To observe the correlation between serum vascular endothelial growth factor, bone mineral density and the number of osteoblasts in the ovariectomized rats.

METHODS: Forty female Sprague-Dawley rats were randomly divided into ovariectomized group and control group. After 3 months, the bone mineral density of the whole body, femur and lumbar spine was measured. Rat enzyme-linked immunosorbent assay kit was used to measure the level of serum vascular endothelial growth factor. Then, the rats in two groups received femoral metaphyseal fixation, decalcified, dehydrated, embeding in paraffin, slicing and hematoxylin-eosin staining. Each slice was free to take five fields of view (10×40) in order to count the osteoblasts of femur distal metaphysis under optical microscope.

RESULTS AND CONCLUSION: After ovariectomized for 3 months, the rats body mass was increased significantly (P < 0.05), and the bone mineral density of the whole body, femur and lumbar spine in the ovariectomized group was lower than that in the control group (P < 0.05), indicating the successful establishment of osteoporosis model. There was no significant difference in vascular endothelial growth factor level between the ovariectomized group and the control group (P > 0.05), and the difference of the osteoblast number between ovariectomized group and control group was not significant (P > 0.05). This indicated that there was no correlation between bone mineral density and the number of osteoblasts and vascular endothelial growth factor level in the ovariectomized group and the control group. These findings suggest that the bone mineral density is reduced and the body mass is increased in the ovariectomized rats, and the reduced bone mineral density of ovariectomized rats may be irrelevant with the change of serum vascular endothelial growth factor.

Key words: tissue construction, bone tissue construction, ovariectomized rat, osteoporosis, vascular endothelial growth factor, osteoblasts, bone mineral density, femoral metaphysic, provincial grants-supported paper

CLC Number:

R318

Bao Xiao-ming, Wang Yun, Hou Yong-xin, Li Jun, Zhang Min, Wei Xiao-chun. Correlation between bone mineral density and serum vascular endothelial growth factor levels in ovariectomized rats[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(28): 5113-5119.

Figures/Tables 3

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