Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (22): 3457-3463.doi: 10.12307/2024.489
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Wang Lu, Xu Jie, Xia Yijing, Zhang Xinsong, Zhao Bin
Received:
2023-08-02
Accepted:
2023-09-25
Online:
2024-08-08
Published:
2024-01-19
Contact:
Zhao Bin, Professor, Doctoral supervisor, School and Hospital of Stomatology of Shanxi Medical University, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, Shanxi Province, China
About author:
Wang Lu, MD, Lecturer, School and Hospital of Stomatology of Shanxi Medical University, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, Shanxi Province, China
Xu Jie, Master candidate, School and Hospital of Stomatology of Shanxi Medical University, Shanxi Province Key Laboratory of Oral Diseases Prevention and New Materials, Taiyuan 030001, Shanxi Province, China
Supported by:
CLC Number:
Wang Lu, Xu Jie, Xia Yijing, Zhang Xinsong, Zhao Bin. Preparation and characterization of silk fibroin/bioactive glass composite fiber membrane[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(22): 3457-3463.
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2.2 各组纤维膜结构表征结果 通过傅里叶红外光谱和X射线衍射分析纤维膜的晶体结构和分子构象,见图2。傅里叶红外光谱显示,5组纤维膜均在1 230 cm-1(酰胺Ⅲ)、1 516 cm-1(酰胺Ⅱ)、1 624 cm-1(酰胺Ⅰ)附近处显示出SF的特征峰,表明经过乙醇处理后的纤维膜表现出典型的Silk Ⅱ结构。添加BG后,可以观察到在1 060 cm-1处出现BG的特征峰(Si-O-Si振动),且随着BG含量的增加而不断增强。X射线衍射分析显示,5组纤维膜均在20.7°处出现明显的衍射峰,表现出典型的Silk Ⅱ结构,此结果与傅里叶红外光谱相一致;同时观察到随着BG含量的增加,纤维膜在15°-30°范围处出现明显的宽且弥散的无定形衍射峰,显示出典型的非晶态聚合物的衍射模式。"
随着培养时间的延长,各组纤维膜上附着的细胞都在稳定增殖,没有表现出明显的细胞毒性。随着纤维膜中BG添加量的增加,细胞增殖吸光度值增加;然而当BG添加量过多时,细胞增殖吸光度值下降,表明添加适量BG的复合纤维膜可以促进骨髓间充质干细胞的增殖。此外还发现,相较于SF组、SF/8BG纤维膜,SF/1BG、SF/3BG、SF/5BG纤维膜均可有效促进骨髓间充质干细胞的生长,其中SF/5BG纤维膜的促细胞增殖能力最佳。 2.7 各组纤维膜对骨髓间充质干细胞活力的影响 培养3 d,各组纤维膜表面均有细胞生长,且未见明显的死细胞;培养7 d,各组纤维膜表面的细胞数量增多,表明材料对于细胞生长无明显毒性作用,具有良好的生物相容性,见图7。相较于其他4组,SF/5BG组纤维膜表面细胞数量相对较多且分布均匀,没有明显的细胞团聚现象,表明添加一定量的BG有利于细胞增殖,该结果与CCK-8检测结果一致。此外,还可见各组纤维膜上的细胞具有一定的形态,呈现多边形形状,且有明显的伪足生长,表明所制备的纤维膜可以有效促进细胞的黏附、生长和增殖。"
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