Chinese Journal of Tissue Engineering Research ›› 2024, Vol. 28 ›› Issue (23): 3627-3635.doi: 10.12307/2023.968
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Zheng Jianbin1, Lu Yuchun1, Jiang Zixian1, Li Xiufang1, Wang Tao2, Wang Wenjing1
Received:
2022-11-29
Accepted:
2023-01-16
Online:
2024-08-18
Published:
2023-09-13
Contact:
Wang Wenjing, MD, Professor, Master’s supervisor, Yunnan University of Chinese Medicine, Kunming 650500, Yunnan Province, China
Wang Tao, Master, Associate chief physician, Department of Orthopedics, Kunming Municipal Hospital of Traditional Chinese Medicine, Kunming 650051, Yunnan Province, China
About author:
Zheng Jianbin, Master candidate, Yunnan University of Chinese Medicine, Kunming 650500, Yunnan Province, China
Supported by:
CLC Number:
Zheng Jianbin, Lu Yuchun, Jiang Zixian, Li Xiufang, Wang Tao, Wang Wenjing. Yi medicine Sambucus adnata Wall methanol extract in a rat osteoarthritis model: therapeutic effect and target prediction[J]. Chinese Journal of Tissue Engineering Research, 2024, 28(23): 3627-3635.
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2.5 SAW-C对骨关节炎模型大鼠血清氧化应激指标的影响 与假手术组相比,模型组血清中前列腺素E2水平明显升高(P < 0.05),与模型组相比,SAW-C高剂量组(P < 0.05)与低剂量组(P < 0.01)大鼠血清中前列腺素E2水平均明显降低,见图5A。与假手术组相比,模型组血清中超氧化物歧化酶水平明显降低(P < 0.05),与模型组相比,SAW-C高、低剂量组大鼠血清中超氧化物歧化酶水平均显著升高(P < 0.01),见图5B。与假手术组相比,模型组血清中丙二醛水平明显升高(P < 0.05),与模型组相比,SAW-C高剂量组大鼠血清中丙二醛水平显著降低(P < 0.01),低剂量组大鼠血清中丙二醛水平仅具有下降趋势但无显著差异,见图5C。"
2.6 苏木精-伊红染色结果 假手术组大鼠关节腔结构完整,软骨表面光滑平整,胫骨平台软骨层厚薄均一,潮线清晰结构完整,软骨细胞排列整齐,层次分明;模型组大鼠关节胫骨平台软骨磨损严重,软骨被大面积结缔组织所取代(蓝色箭头),可见大量炎症细胞浸润(红色箭头),软骨全层破坏,软骨细胞排列紊乱,大量集簇于软骨组织表面并且数量明显减少,潮线结构消失;与模型组相比,布洛芬组大鼠关节胫骨平台软骨表面相对平滑,但仍然有轻微磨损,软骨层厚度稍不均一,潮线结构相对完整,软骨细胞排列稍显紊乱;SAW-C低剂量组大鼠关节胫骨平台软骨磨损情况得到改善,表面相对完整光滑,潮线相对结构完整但不清晰,软骨细胞排列与数量如常;SAW-C高剂量组大鼠关节胫骨平台软骨表面平滑,产生微小裂隙,潮线结构不清晰,软骨细胞排列与数量如常,提示SAW-C能够维持骨关节炎大鼠膝关节软骨形态,缓解骨关节炎的发展,见图6。"
2.9 SAW-C对透明软骨厚度的影响 与假手术组相比,模型组透明软骨厚度显著降低(P < 0.001),与模型组相比,SAW-C高、低剂量组透明软骨厚度均显著升高(P < 0.001),见图8。 2.10 SAW-C对膝关节软骨矿质化程度的影响 与假手术组相比,模型组大鼠膝关节软骨矿质化程度显著升高(P < 0.001),与模型组相比,SAW-C高、低剂量组大鼠膝关节软骨矿质化程度均显著下降(P < 0.001),见图8。 2.11 SAW-C对软骨下骨骨小梁面积的影响 与假手术组相比,模型组大鼠膝关节软骨下骨骨小梁面积显著减小(P < 0.001),与模型组相比,SAW-C高、低剂量组大鼠膝关节软骨下骨骨小梁面积均显著上升(P < 0.05),见图9。"
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