Screening and verification of differentially expressed genes in inflammatory tissues with peri-implantitis

doi:10.12307/2023.802

Abstract

Abstract: BACKGROUND: Peri-implantitis seriously affects the success of dental implant restorations. There is still no effective cure and its pathogenesis has not yet been elucidated.
OBJECTIVE: To find key differentially expressed genes related to the occurrence of peri-implantitis and experimentally verify them.
METHODS: Differentially expressed genes between the peri-implantitis and the healthy gums were screened from the GEO database using bioinformatics technology and were analyzed by enrichment analysis. A protein interaction network was constructed using the String database. Key genes were screened by Cytoscape software. Expression of key gene C3AR1 was validated using RT-PCR and immunohistochemistry. The expression level of C3AR1 mRNA in RAW264.7 cells after osteoclast differentiation was detected by RT-PCR.
RESULTS AND CONCLUSION: (1) The gene data of peri-implantitis and the healthy gums in the GSE33774 data set were analyzed for differences. A total of 50 differentially expressed genes were obtained, including 33 upregulated genes and 17 downregulated genes. GO functional enrichment and KEGG pathway enrichment analysis showed that differentially expressed genes mainly played a role in immune receptor activity, C-C chemokine receptor activity, and the signaling pathway of osteoclast differentiation. (2) Six key target genes were identified based on the protein interaction network, including C3AR1, CD14, TLR4, CCR1, CYBB, and FCGR2A. The results of RT-PCR and immunohistochemistry experiments showed that the expression of C3AR1 at mRNA and protein levels was significantly increased in peri-implantitis tissue. Moreover, C3AR1 was upregulated after osteoclastic differentiation of macrophages. (3) It is concluded that C3AR1 showed high expression in peri-implantitis, which may be a key gene for bone destruction in peri-implantitis.

Key words: peri-implantitis, C3AR1, bioinformatics, differentially expressed gene, key gene, mononuclear macrophage, osteoclast differentiation, bone destruction

CLC Number:

Wei Yuxing, Dong Hao, Wei Huiping, Xie Cheng, Tuo Yangjuan, Qin Hao, Cao Yong. Screening and verification of differentially expressed genes in inflammatory tissues with peri-implantitis[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(30): 4844-4849.

Figures/Tables 7

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