Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (29): 4598-4604.doi: 10.12307/2022.881
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Wu Juhua1, Yang Yanan2, Weng Xiquan3, Zhao Fangfang4, Xu Guoqin3, Lin Wentao3, 4
Received:
2021-03-27
Accepted:
2021-05-09
Online:
2022-10-18
Published:
2022-03-27
Contact:
Lin Wentao, Professor, Department of Sports and Health, Guangzhou Sport University, Guangzhou 510500, Guangdong Province, China; Zhuhai College of Science and Technology, Zhuhai 519000, Guangdong Province, China
About author:
Wu Juhua, MD, Associate professor, School of Physical Education, Guangxi University of Science and Technology, Liuzhou 545006, Guangxi Zhuang Autonomous Region, China
Supported by:
CLC Number:
Wu Juhua, Yang Yanan, Weng Xiquan, Zhao Fangfang, Xu Guoqin, Lin Wenta. Effects of hypoxic exercise on skeletal muscle energy metabolism of rats with alimentary obesity[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(29): 4598-4604.
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2.1 实验动物数量分析 干预结束后,剩余大鼠55只,其中常氧安静组和16.3%低氧安静组均为10只;常氧运动组、16.3%低氧运动组以及13.3%低氧安静组均为9只;13.3%低氧运动组为8只。实验中共脱失5只大鼠,其中常氧运动组1只、16.3%低氧运动组1只和13.3%低氧运动组2只均因运动时颈部折断而死亡,13.3%低氧安静组1只则因长有肿物而死亡。在进行蛋白测定时,根据G power软件进行样本量计算,得出每组n≥6即达到所需效应量。 2.2 高脂膳食诱导营养性肥胖大鼠模型建立[3] 7周高脂膳食饲养后,高脂膳食造模组大鼠的体质量、体长、体质量指数均高于普通膳食组[12],差异有非常显著性意义(P < 0.01),见表1。"
综上,7周高脂膳食饲养后,高脂膳食造模组大鼠形态学变化表现为体质量、体质量指数、体长显著高于普通膳食组,且体质量增长高于普通膳食组大鼠20%;血液生化相关指标表现为血糖、总胆固醇、低密度脂蛋白胆固醇、三酰甘油水平显著高于普通膳食组,提示高脂膳食诱导营养性肥胖大鼠模型建立成功[11]。 2.3 低氧运动对营养性肥胖大鼠骨骼肌细胞PGC-1α蛋白表达的影响[3] 常氧安静组和常氧运动组、常氧安静组和16.3%低氧运动组、常氧运动组和13.3%低氧运动组、16.3%低氧安静组和16.3%低氧运动组、13.3%低氧安静组和13.3%低氧运动组大鼠骨骼肌PGC-1α蛋白表达水平相比差异均有显著性意义(P < 0.05);常氧安静组和13.3%低氧运动组、16.3%低氧安静组和13.3%低氧运动组大鼠骨骼肌PGC-1α蛋白表达水平相比差异有非常显著性意义(P < 0.01),见表3。"
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