Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (7): 1142-1148.doi: 10.3969/j.issn.2095-4344.2183

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Efficacy of mesenchymal stem cells in the spinal cord injury of large animal models: a meta-analysis

Kong Desheng1, 2, He Jingjing1, 2, Feng Baofeng1, 2, Guo Ruiyun1, 2, Asiamah Ernest Amponsah1, 2, Lü Fei1, 2, Zhang Shuhan1, 2, Zhang Xiaolin3, Ma Jun1, 2, 4, Cui Huixian1, 2, 4   

  1. 1Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Shijiazhuang 050017, Hebei Province, China; 2Hebei Research Center for Stem Cell Medical Translational Engineering, Shijiazhuang 050017, Hebei Province, China; 3Department of Epidemiology and Health Statistics, 4Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China
  • Received:2020-04-20 Revised:2020-04-23 Accepted:2020-05-28 Online:2021-03-08 Published:2020-12-09
  • Contact: Ma Jun, MD, Associate professor, Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Shijiazhuang 050017, Hebei Province, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Shijiazhuang 050017, Hebei Province, China; Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China Cui Huixian, MD, Professor, Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Shijiazhuang 050017, Hebei Province, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Shijiazhuang 050017, Hebei Province, China; Department of Anatomy, Hebei Medical University, Shijiazhuang 050017, Hebei Province, China
  • About author:Kong Desheng, MD, Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Shijiazhuang 050017, Hebei Province, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Shijiazhuang 050017, Hebei Province, China
  • Supported by:
    the High-Level Talent Funding Project in Hebei Province, No. B2019005009; the Youth Program of National Natural Science Foundation of China, No. 81801278; the General Program of Natural Science Foundation of Hebei Province, No. H2019206637

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Abstract: OBJECTIVE: Mesenchymal stem cells transplantation is a promising treatment for spinal cord injury. Most of studies focused on small animal models. In large animal experiments, there are still controversies in selection of stem cells and therapeutic effect. This article analyzed the effects of mesenchymal stem cells on related indicators of spinal cord injury in large animal models and evaluated their effects on spinal cord injury repair.
METHODS: PubMed, Cochrane, OVID, Web of Science and CNKI databases were retrieved before December 2019. A series of studies on the treatment of spinal cord injury in large animal models by mesenchymal stem cells were collected. According to the inclusion criteria, two researchers independently completed literature screening, data extraction and methodological quality evaluation, and meta-analysis was conducted with Stata16.0.  
RESULTS:  A total of 10 articles were included. The results of meta-analysis showed that: (1) Mesenchymal stem cells could significantly improve motor function after spinal cord injury [I2=97.73%, MD=3.94, 95%CI (2.15, 5.72), P < 0.01]. Based on cell source, observation time, intervention phase, transplantation mode and graft type subgroup analysis showed that motor scores of bone marrow mesenchymal stem cells group, non-bone marrow mesenchymal stem cells group, short-term observation (< 2 months) group, long-term observation (≥ 2 months) group, acute stage group, non-acute stage group, cells group and allograft group were significantly higher than those of control group (P < 0.01). There was no significant difference in motor score between scaffold group and control group (P > 0.05). (2) The injury size in mesenchymal stem cells treatment group was significantly smaller than that in the control group [I2=98.05%, MD=-1.00, 95%CI (-1.95, -0.04), P=0.04]. (3) There was no significant difference in the relative expression of glial fibrillary acidic protein between the mesenchymal stem cells treatment group and the control group [I2=99.48%, MD=80.61, 95%CI (-27.48, 188.70), P=0.14]. 
CONCLUSION: Mesenchymal stem cells transplantation has a significant improvement on the motor function and injury repair of spinal cord injury in large animals, and the security is high. Due to the limitation of the quality of the included literature, the above conclusions need to be validated by high-quality and large-sample randomized controlled trials.

Key words: stem cells, mesenchymal stem cells, spinal cord injury, large animals, experiment, motor function, protein, meta-analysis

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