Influence of collagen coating on the biocompatibility of three-dimensional printed implants

doi:10.3969/j.issn.2095-4344.2017.10.014

Abstract

Abstract:

BACKGROUND: Inert biomaterials such as metal usually hold poor biocompatibility and weak bonding force, which is against the effect of dental or bone implants. Therefore, how to improve their biocompatibility has become the research hotspot.
OBJECTIVE: To prepare collagen-coated titanium alloy (Ti6Al4V), and to assess its biocompatibility.
METHODS: Ti6Al4V served as the matrix, the 3-amino propyl triethoxy silane (KH550) as the crosslinking agent, and the three-dimensional printed titanium alloy coated by collagen type I and II was prepared, respectively. The coated materials were co-cultured with mouse preosteoblasts MC-3T3-E1 to evaluate its biocompatibility. The effect of different kinds of collagen on the cell differentiation was compared by differential recognition of surface proteins.
RESULTS AND CONCLUSION: When the N content on the titanium alloy surface (Φ10 cm) was 8.41%, the cladding quantity of collagen type I and II was 0.81 and 0.77 mg, respectively. Compared with the bare titanium alloy, the cell adhered well and distributed extensively on the coated titanium alloy, which showed strong viability and fast proliferation. The cells cultured on collagen type I coated materials expressed the proteins associated with matrix synthesis, and those on collagen type II coated materials expressed the proteins associated with mineralization. These results clarify that the collagen coating can improve the biocompatibility of titanium alloy, and different types of collagens act on different functional proteins.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Titanium, Alloys, Collagen, Osteoblasts, Biocompatible Materials, Tissue Engineering

CLC Number:

R318

Li Sai-na, Kang Ji-yao, Gao Jian-ping, Gao Yi, Luo Yuan-ming, Zhang Gui-feng, Wang Ming-lin. Influence of collagen coating on the biocompatibility of three-dimensional printed implants[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(10): 1558-1564.

Figures/Tables 8

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