Influence of persimmon tannin water extract on periodontal ligament fibroblast proliferation

doi:10.3969/j.issn.2095-4344.2015.51.012

Abstract

Abstract:

BACKGROUND: Persimmon is rich in tannin substances. Tannin has antibacterial, antiviral, antioxidant, anti-tumor effects as well as protects the mucosa and has a role of convergence, and its practicality and safety in the mouth have been certified.
OBJECTIVE: To investigate the effects of persimmon tannin water extract with different concentrations on the proliferation of periodontal ligament cells.
METHODS: Premolars from adolescents due to orthodontic extraction were placed in a sterile DMEM medium to culture periodontal ligament cells in vitro. Tannin from persimmon fruit was extracted by ultrasound. Cells were cultured in medium containing different concentrations of persimmon tannin water extracts (0.013 3, 0.025 9, 0.038 9, 0.088, 0.147, 0.440, 0 g/L) for 24, 48 and 72 hours, respectively. Then, MTT, coomassie brilliant blue staining and alkaline phosphatase method were used to observe the effects of persimmon tannin water extract on the number of periodontal ligament cells, total protein content and cell viability at different time points, respectively.
RESULTS AND CONCLUSION: At 24, 48 and 72 hours after culture, the persimmon tannin water extract at different concentrations could promote the cell growth, accelerate the protein synthesis, and strengthen the activity in a dose- and time-dependent manner. These findings indicate that the persimmon tannin water extraction can promote the proliferation of human periodontal ligament cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Liu Xiao-li, Liu Wen-xuan, Zhou Xiang, Liu Xin, Yin Wei-wei, Liu Xue-cong.

Influence of persimmon tannin water extract on periodontal ligament fibroblast proliferation

[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(51): 8266-8270.

Figures/Tables 4

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