Nrf2-mediated curcumin protects chondrocytes from oxidative stress

doi:10.3969/j.issn.2095-4344.2015.51.004

Abstract

Abstract:

BACKGROUND: Several studies have showed that oxidative stress plays an important role in the development of osteoarthritis. Curcumin has the effect of antioxidation and eliminating free radicals.
OBJECTIVE: To observe the protective effect of curcumin on chondrocytes through the establishment of a model of oxidative stress damage induced by H2O2.
METHODS: Sprague-Dawley rat articular chondrocytes were prepared and cultured in vitro. Then, the cells were randomly divided into five groups: normal, model (H2O2), low-, mediate-, high-dose curcumin groups (20, 40, 80 μmol/L). Curcumin and chondrocytes were incubated about 48 hours, and then H2O2 was added and co-cultured for 24 hours. Cell viability was detected by MTT assay, and the levels of superoxide dismutase, malondialdehyde, catalase in cells were detected. The mRNA and protein expressions of Nrf2 were detected by real-time PCR and western blot, respectively. The changes of cell nucleus shape were observed.
RESULTS AND CONCLUSION: Compared with the normal group, in the model group, the survival rate of chondrocytes decreased; the levels of superoxide dismutase and catalase decreased, while the level of malondialdehyde increased; the mRNA and protein expressions of Nrf2 were down-regulated, and the
characteristics of apoptotic cells were presented. Compared with the model group, in the three groups pretreated with curcumin, the survival rate of chondrocytes increased; the levels of superoxide dismutase and catalase increased, while the level of malondialdehyde decreased; the mRNA and protein expressions of Nrf2 were up-regulated, and the morphology of cell nucleus was improved by curcumin pretreatment. With the increase of concentration, these changes were more obvious. These findings indicate that the protective effect of curcumin against oxidative stress injury induced by H2O2 in chondrocytes can be achieved by enhancing the expression of Nrf2.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Song Yong-zhou, Guan Jian, Li Ming, Ma Wei, Yuan He, Wang Bin, Tong Jiu-hui. Nrf2-mediated curcumin protects chondrocytes from oxidative stress[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(51): 8218-8222.

Figures/Tables 4

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