Construction of elderly SIRT1 gene knockout mouse models of knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2015.49.005

Abstract

Abstract:

BACKGROUND: Scholars in China and countries outside China conducted a lot of researches on the pathogenesis and treatment of osteoarthritis, and recently found that SIRT1 gene has an important significance in the pathogenesis of osteoarthritis. However, little is reported on SIRT1 gene, and there are few studies on animal models of knee osteoarthritis used for SIRT1 research.
OBJECTIVE: To establish specific SIRT1 gene knockout mouse models of knee osteoarthritis, in order to provide favorable conditions for the study of osteoarthritis.
METHODS: Mice were randomly divided into four groups according to the method of randomized controlled grouping: SIRT1+/+ control group (Group A, n=6); SIRT1+/+ osteoarthritis model group (Group B, n=6); SIRT1-/- control group (Group C, n=6); SIRT1-/- osteoarthritis model group (Group D, n=6). Unilateral knee anterior cruciate ligament transection and medial meniscectomy were conducted to establish mouse models of osteoarthritis. SIRT1 gene knockout was determined by quantitative fluorescence polymerase chain reaction. Knee cartilage morphological changes were observed by hematoxylin-eosin and safranin O-fast green double staining. The degree of knee osteoarthritis was evaluated by Mankin score.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Yu Fei, Lei Ming, Zeng Hui, Li Ming-hua, Xiao De-ming. Construction of elderly SIRT1 gene knockout mouse models of knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7895-7901.

Figures/Tables 4

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