Biomechanical properties of a decellularized scaffold of lyophilized bovine tendon

doi:10.3969/j.issn.2095-4344.2015.30.021

Abstract

Abstract:

BACKGROUND: Current decellularized methods have the certain damage to the extracellular matrix and reduce the biomechanical properties of acellular scaffolds.
OBJECTIVE: To explore the biomechanical properties of decellularized scaffold of lyophilized bovine tendon.
METHODS: Sixty lyophilized fiber bundles from fresh flexion tendon of calf toes were randomly divided into two groups: control group and experimental group. In the experimental group, serine protease inhibitors were placed aseptically for 24 hours at room temperature, then the samples were rinsed with PBS and transferred to the low concentration of trypsin+ethanol mixed solution to remove the cell wall without destruction of the extracellular matrix at room temperature for 5 hours; after that, the fiber bundles were cultured in DNA enzyme solution for 5 hours, finally the acellular scaffold was completed and rinsed with PBS for 48 hours and dried at room temperature in sterile room. No treatment was done in the control group. Modulus of elasticity, durability and maximum stress were determined in the two groups.
RESULTS AND CONCLUSION: Similar elastic modulus and durability were found in the two groups, but the maximum stress in the experimental group was significantly lower than that in the control group (P < 0.01). These findings indicate that the lyophilized acellular tendon fibers can mimic the biological function of bovine tendon
fibers to a certain extent.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

CLC Number:

R318

Qian Chuang, Chen Xiong-sheng, Zhou Sheng-yuan, Zhu Wei. Biomechanical properties of a decellularized scaffold of lyophilized bovine tendon[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(30): 4865-4869.

Figures/Tables 2

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