Chinese Journal of Tissue Engineering Research ›› 2025, Vol. 29 ›› Issue (17): 3605-3613.doi: 10.12307/2025.638

Previous Articles     Next Articles

Effects of 1,8-cineole on inflammatory response in a rat model of experimental periodontitis

He Li1, 2, Ren Lu1, 2, Jiang Xiaoxi1, 2, Liu Xuqian1, 2, Li Chunhui1, 2   

  1. 1Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Institute of Stomatology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2024-05-21 Accepted:2024-07-20 Online:2025-06-18 Published:2024-11-02
  • Contact: Liu Xuqian, MD, Associate professor, Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Institute of Stomatology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:He Li, Master candidate, Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Institute of Stomatology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China Corresponding author: Li Chunhui, MS, Associate professor, Department of Periodontics & Oral Mucosal Diseases, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Institute of Stomatology, The Affiliated Stomatological Hospital, Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:
    the State Key Laboratory of Oral Disease Prevention and Treatment Open Project, No. SKLOD2024OF04 (to LXQ); Sichuan Province Medical Scientific Research Project Plan, No. S23043 (to LXQ); the Science and Technology Innovation Talent Project of Luzhou City, No. 2023RCX171 (to LXQ); the “RYTIME Foundation” Scientific Research and Cultivation Program of the Affiliated Stomatological Hospital of Southwest Medical University, No. 2022RT07 (to HL)

PDF

147

Abstract: BACKGROUND: Previous studies have shown that 1,8-cineole has anti-inflammatory, antioxidation, antibacterial and anti-tumor effects. It has good anti-inflammatory effects in many diseases. 
OBJECTIVE: To investigate the effect of 1,8-cineole on inflammatory response in a rat model of experimental periodontitis.
METHODS: Thirty Sprague-Dawley rats were assigned into normal control group, periodontitis control group and 1,8-cineole group with ten rats in each group according to the completely randomized digital table method. Except for the normal control group, rats in the other groups were induced into experimental periodontitis. The periodontitis model was constructed by the orthodontic ligature wire method. Eight weeks after modeling, in the 1,8-cineole group, 1,8-cineole was placed into periodontal pockets, twice per day for 4 weeks. In the normal control group and the periodontitis control group, the same amount of normal saline was placed into periodontal pockets, twice per day for 4 weeks. After administration, general observation and periodontal clinical indicators were performed. Hematoxylin-eosin staining was used for periodontal histological evaluation. The expressions of inflammatory factors in the serum and gingiva at mRNA and protein levels were detected.
RESULTS AND CONCLUSION: (1) Compared with the normal control group, rats in the periodontitis control group showed increased gingival bleeding index and periodontal probing depth (P < 0.05), increased serum levels of interleukin 1β, tumor necrosis factor α, and interleukin 6 (P < 0.05), decreased serum level of interleukin 10 (P < 0.05), increased mRNA and protein levels of interleukin 1β, tumor necrosis factor α, and interleukin 6 in gingival tissue (P < 0.05), and decreased mRNA and protein level of interleukin 10 in gingival tissue (P < 0.05). Hematoxylin-eosin staining of periodontal tissues showed that compared with the normal control group, periodontal inflammation was obvious in the periodontitis control group. (2) Compared with the periodontitis control group, rats in the 1,8-cineole group showed decreased gingival bleeding index and periodontal probing depth (P < 0.05), decreased serum levels of interleukin 1β, tumor necrosis factor α, and interleukin 6 (P < 0.05), increased serum level of interleukin 10 (P < 0.05), decreased mRNA and protein levels of interleukin 1β, tumor necrosis factor α, and interleukin 6 in gingival tissue (P < 0.05), and increased mRNA and protein level of interleukin 10 in gingival tissue (P < 0.05). Hematoxylin-eosin staining of periodontal tissues showed that compared with the periodontitis control group, periodontal inflammation was remarkably alleviated in the 1,8-cineole group. To conclude, 1,8-cineole can attenuate the inflammatory response in the rat model of experimental periodontitis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: 1,8-cineole, plant essential oil, periodontitis, animal model, periodontal tissue, inflammatory response, interleukin 1β, tumor necrosis factor α, interleukin 6, interleukin-10, engineered tissue construction

CLC Number: