Platelet-derived growth factor BB induces bone marrow mesenchymal stem cells to differentiate into vascular endothelial cells

doi:10.12307/2021.001

Abstract

Abstract: BACKGROUND: There are few reports about platelet-derived growth factor BB promoting angiogenesis of bone marrow mesenchymal stem cells. However, the nutrition, growth factors and cytokines provided by neovascular network play an important role in bone tissue formation.
OBJECTIVE: To investigate the effects of platelet-derived growth factor-BB on the differentiation of bone marrow mesenchymal stem cells of Sprague-Dawley rats into vascular endothelial cells.
METHODS: (1) Bone marrow mesenchymal stem cells of Sprague-Dawley rats were isolated and cultured in vitro. The surface markers CD45, CD29 and CD34 of the third generation were identified by flow cytometry. Bone marrow mesenchymal stem cells with high purity were selected for platelet-derived growth factor BB intervention experiments. (2) Passage 3 bone marrow mesenchymal stem cells were induced by 25 μg/L platelet-derived growth factor BB for 3 days. Matrigel was selected for cell lumen formation experiments. The immunofluorescence method was used to detect the expression of FITC-UEA-1 and DIL-ac-LDL in cells to verify the fluorescence expression of CD31 in cells. Flow cytometry was used to detect the expression of CD31 in cells after induction. Finally, the mRNA level of vascular endothelial growth factor gene was detected by RT-PCR.
RESULTS AND CONCLUSION: The cells were mixed with Matrigel and inoculated after platelet-derived growth factor BB induction. A clear tubular structure was formed; the expressions of FITC-UEA-1 and DIL-ac-LDL and CD31 expression were positive in bone marrow mesenchymal stem cells after induction. The expression of VEGF mRNA in platelet-derived growth factor BB-induced cells was higher than that in bone marrow mesenchymal stem cells without platelet-derived growth factor BB (P < 0.05). It is concluded that rat bone marrow mesenchymal stem cells can differentiate into functional vascular endothelial cells after being induced by platelet-derived growth factor BB.

Key words: stem cells, bone marrow mesenchymal stem cells, platelet derived growth factor, vascular endothelial cells, flow cytometry, rat

CLC Number:

Jiang Tao, Ma Lei, Li Zhiqiang, Shou Xi, Duan Mingjun, Wu Shuo, Ma Chuang, Wei Qin. Platelet-derived growth factor BB induces bone marrow mesenchymal stem cells to differentiate into vascular endothelial cells[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(25): 3937-3942.

Figures/Tables 8

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