Expression of bone growth factor in rats with infectious bone defects after treatment with bone migration

doi:10.3969/j.issn.2095-4344.2857

Abstract

Abstract:

BACKGROUND: Studies have shown that bone healing is a pathophysiological process that completes repair, regeneration and reconstruction in which multiple growth factors are involved. Insulin-like growth factors-1

(IGF-1) and basic fibroblast growth factor (bFGF) are two important bone growth factors in the process of bone healing.

OBJECTIVE: To investigate the expression of IGF and bFGF before and after bone migration in rats with infectious bone defects.

METHODS: Thirty-six Sprague-Dawley rats were selected, and then each rat was made an infectious bone defect of 4 mm long at the lower end of tibia. After 2 weeks, the rats were randomly divided into two groups: an operation group and a control group. After debridement, stents were installed with no bone migration in the control group, and bone migration was performed in the operation group. X-ray observation was performed at the 2^nd week after stenting. The healing of infectious bone defects and the expression of IGF and b-FGF in the two groups were detected by hematoxylin-eosin staining and enzyme-linked immunosorbent assay at the 2^nd, 3^rdand 4^th weeks. The experimental protocol was approved by the Animal Experimental Ethics Committee of Guangxi Medical University (approval No. 201903036).

RESULTS AND CONCLUSION: X-ray findings showed callus formation in the defect area at the 2^ndweek postoperatively, and no swelling in the surrounding soft tissue with good healing effects. Hematoxylin-eosin staining showed less fibrous tissues, more osteoblasts, denser trabeculae, and more mesenchymal cells and new capillaries in the operation group than the control group. Enzyme-linked immunosorbent assay findings showed that the expression of IGF-1 was significantly increased in the operation group compared with the control group in the 2^nd and 3^rdweeks postoperatively (P < 0.05); the expression of bFGF was significantly different from that in the control group at the 2^nd week postoperatively (P < 0.05). In conclusion, IGF-1 and b-FGF is up-regulated in rats after bone migration, which suggests that the up-regulation of IGF-1 and bFGF may be one of the reasons for promoting the healing of infectious bone defects.

Key words: bone, bone defect, growth factor, infectious bone defect, model, mouse, experiment

CLC Number:

Guo Yande, Zeng Gaofeng, Wei Shoufeng, Zhang Qiong, Zhou Quan, Zhang Chuanyang, Zong Shaohui. Expression of bone growth factor in rats with infectious bone defects after treatment with bone migration[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(32): 5102-5107.

Figures/Tables 5

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