Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (17): 2718-2723.doi: 10.3969/j.issn.2095-4344.2557
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Xie Mingzhong, Li Sen, Zhu Kai, Jiang Huacai, Qi Lisheng
Received:
2019-07-03
Revised:
2019-07-04
Accepted:
2019-08-09
Online:
2020-06-18
Published:
2020-03-30
Contact:
Li Sen, MD, Professor, Department of Orthopedics and Traumatology, Affiliated Hospital of Traditional Chinese Medicine, Southwest Medical University, Luzhou 646600, Sichuan Province, China
About author:
Xie Mingzhong, Master, Attending physician, Department of Orthopedics and Traumatology, Affiliated Hospital of Traditional Chinese Medicine, Southwest Medical University, Luzhou 646600, Sichuan Province, China
Supported by:
CLC Number:
Xie Mingzhong, Li Sen, Zhu Kai, Jiang Huacai, Qi Lisheng. Expression of cyclic RNA plasmacytoma variant translocation 1 in senescence of nucleus pulposus cells and its regulation mechanism[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(17): 2718-2723.
2.2 circPVT1、let-7在衰老髓核细胞中的表达 与正常髓核细胞相比,辐射组细胞中circPVT1 mRNA表达明显降低(1.05±0.04,0.38±0.07),而let-7 mRNA表达明显升高(1.09±0.06,1.59±0.38),差异有显著性意义(P < 0.05)。 2.3 共转染后各组髓核细胞circPVT1、let-7的表达 与空白组、si-NC+anti-NC组相比,si-circPVT1+anti-NC组circPVT1 mRNA表达降低,let-7 mRNA表达升高,si-NC+anti-let-7组circPVT1 mRNA表达升高,let-7 mRNA表达降低(P < 0.05)。si-circPVT1+anti-let-7组circPVT1 mRNA表达高于si-circPVT1+anti-NC组,低于si-NC+anti-let-7组;let-7 mRNA表达低于si-circPVT1+anti-NC组,高于si-NC+anti- let-7组(P < 0.05),见表1。 "
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