Abstract:

BACKGROUND: Platelet-rich plasma is an autogeneic extract containing and releasing a variety of growth factors, which has been used in the research of bone and cartilage tissue engineering and regeneration.
OBJECTIVE: To investigate the preparation methods of platelet-rich plasma and its influencing factors by comparing the platelet concentration in the platelet-rich plasma prepared by different methods in rabbits and detecting the levels of platelet-derived growth factor and transforming growth factor β1.
METHODS: Platelet-rich plasma was prepared by the methods of Petrungaro, Landesberg and Aghaloo. Platelet amount was counted in the platelet-rich plasma prepared using three methods as well as platelet-derived growth factor and transforming growth factor β1 levels.
RESULTS AND CONCLUSION: There were significant differences in the platelet count, platelet recovery rate and platelet enrichment coefficient in the platelet-rich plasma prepared by these three methods (P < 0.001). The effective concentration of platelet-rich plasma could be prepared by the methods of Landesberg and Aghaloo, and the platelet concentration and activity prepared by Aghaloo method was higher than that prepared by Landesberg method with a statistical difference (P < 0.05). The levels of platelet-derived growth factor and transforming growth factor β1 in the inactivated platelet-rich plasma were not statistically different from those in the normal plasma and platelet-poor plasma. The levels of platelet-derived growth factor and transforming growth factor β1 in the activated platelet-rich plasma prepared by Landesberg and Aghaloo methods were significantly higher than before activation (P < 0.001), especially in the activated platelet-rich plasma prepared by Aghaloo method (P < 0.05). The platelet count in the platelet-rich plasma was positively correlated with the levels of platelet-derived growth factor (r=0.872, P < 0.001) and transforming growth factor β1 (r=0.917, P < 0.001).

Key words: biomaterials, basic experiments of biomaterials, platelet-rich plasma, plasma, platelet, platelet-derived growth factor, transforming growth factor, platelet activation, cartilage, activation, tissue engineering, tissue regeneration, other grants-supported paper, biomaterial photographs-containing paper