Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (41): 7717-7722.doi: 10.3969/j.issn.2095-4344.2012.41.024

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Tougu Xiaotong Granule water/alcohol extract-containing serum interferes the activity of bone marrow stromal stem cells

Liu Bo-ling1, Li Xi-hai2, Liang Gui-qing1, Chen Qi-yong1, Wu Guang-wen2, Li Zhao-hui1   

  1. 1Department of Orthopedics, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine (Second Hospital of Fuzhou), Fujian University of Traditional Chinese Medicine, Fuzhou 350007, Fujian Province, China
    2Academy of Integrated Traditional Chinese and Western Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, Fujian Province, China
  • Received:2012-02-05 Revised:2012-03-19 Online:2012-10-07 Published:2012-10-07
  • About author:Liu Bo-ling☆, M.D., Attending physician, Department of Orthopedics, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine (Second Hospital of Fuzhou), Fujian University of Traditional Chinese Medicine, Fuzhou 350007, Fujian Province, China liuboling2000@126.com

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Abstract:

BACKGROUND: Bone marrow stromal stem cells have multilineage differentiation capacity and can differentiate into transparent chondrocytes, which can provide new thoughts for treatment of osteoarthritis.
OBJECTIVE: To investigate the effects of Tougu Xiaotong Granule water/alcohol extract-containing serum on the activities of bone marrow stromal stem cells.
METHODS: Tougu Xiaotong Granule water/alcohol extracts were prepared. A total of 108 SD rats were randomly divided into control group, Tougu Xiaotong Granule water extract group and Tougu Xiaotong Granule alcohol extract group. Then each group was divided into three subgroups as per low, medium, and high dose. Abdominal aorta blood was taken to prepare drug-containing serum. Passage 3 bone marrow stromal stem cells from 4-week-old SD rats were interfered with Tougu Xiaotong Granule water/alcohol extract-containing serum, and then cell activity was detected by MTT. Cell cycle was determined by flow cytometry. Cyclin D1 mRNA expression was detected by real-time PCR. Cyclin D1 protein expression was determined by western blot analysis.
RESULTS AND CONCLUSION: After 48 hours of intervention by Tougu Xiaotong Granule water/alcohol extract, the ratio of cells in the G0/G1 period in the Tougu Xiaotong Granule water extract group and Tougu Xiaotong Granule alcohol extract group was significantly lower than that in the control group (P < 0.01), the ratio of cells in the G0/G1 period in the Tougu Xiaotong Granule water extract group was significantly lower than that in the Tougu Xiaotong Granule alcohol extract group (P < 0.05). Proliferation index and cyclin D1 mRNA and protein expression in the Tougu Xiaotong Granule water extract group were significantly higher than in the control group (P < 0.01), and these indices were significantly higher in the Tougu Xiaotong Granule water extract group than in the Tougu Xiaotong Granule alcohol extract group (P < 0.05). These findings suggest that Tougu Xiaotong Granule water/alcohol extract-containing serum promotes the proliferation of bone marrow stromal stem cells by upregulating Cyclin D1 expression and accelerating the progression of cell cycle; Tougu Xiaotong Granule water extract-containing serum exhibits better effects than Tougu Xiaotong Granule alcohol extract-containing serum.

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