Abstract:

BACKGROUND: Researches showed that oncofetal fibronectin is an important indicator of new synthetic extracellular matrix. But studies of relationship between oxidative stress in high glucose environment and oncofetal fibronection are rarely reported.
OBJECTIVE: To investigate the effect of high glucose on the expression of reactive oxygen species (ROS) and oncofetal fibronectin mRNA in human mesangial cells.
METHODS: The cultured mesangial cells were divided into following groups: normal control group (5 mmol/L D-glucose), osmotic control group (5 mmol/L D-glucose+20 mmol/L L-glucose) and high glucose group (25 mmol/L D-glucose); α-lipoic acid intervention group containing high glucose+α-lipoic acid 50 group (25 mmol/L D-glucose+50 μmol/L α-lipoic acid), high glucose+α-lipoic acid 100 group (25 mmol/L D-glucose+100 μmol/L α-lipoic acid) and high glucose+α-lipoic acid 200 group    (25 mmol/L D-glucose+200 μmol/L α-lipoic acid). The expression of oncofetal fibronectin mRNA was detected by reverse transcription-PCR. ROS levels were tested by fluorescence microscopy and fluorescence microplate reader.
RESULTS AND CONCLUSION: High glucose could promote the production of ROS and increase the expression of oncofetal fibronectin mRNA in the mesangial cells. α-lipoic acid could significantly reduce the level of ROS and expression of oncofetal fibronectin mRNA in the mesangial cells under high glucose loaded, which showed a concentration-dependent manner. It is indicated that ROS mediated high glucose can induce the expression of oncofetal fibronectin in the mesangial cells, but this effect can be partially reversed by α-lipoic acid.