Chinese Journal of Tissue Engineering Research

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Effect of joint synovial fluid on biodynamics and histology of tendon-bone healing after reconstruction of anterior cruciate ligament in rabbits

Dong Xiang-hui, Ling Ming, Feng Wei-lou, Tian Xin   

  1. Second Department of Orthopedics, Institute of Clinical Medicine, Third Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Shaanxi Provincial People’s Hospital, Xi’an  710068, Shaanxi Province, China
  • Received:2011-09-19 Revised:2012-01-18 Online:2012-03-11 Published:2012-03-11
  • Contact: author: Ling Ming, Master, Master’s supervisor, Chief physician, Second Department of Orthopedics, Institute of Clinical Medicine, Third Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China m.ling0114@163.com
  • About author:Dong Xiang-hui★, Master, Second Department of Orthopedics, Institute of Clinical Medicine, Third Affiliated Hospital, School of Medicine, Xi’an Jiaotong University, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China donggege-0829@163.com
  • Supported by:

    13115” Major Science and Technology Innovation Projects of Shaanxi Province in 2008, No. 2008ZDKG-57*

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Abstract:

BACKGROUND: At present, effect of joint synovial fluid on the strength of graft and tendon-bone healing after reconstruction of anterior cruciate ligament (ACL) is inconclusive.
OBJECTIVE: To observe the effect of joint synovial fluid on biodynamics and histology of tendon-bone healing after reconstruction of ACL in the rabbits.
METHODS: Semitendinosus tendons were taken from lower extremities of New Zealand white rabbits to reconstruct ACL simulation synovial fluid influence model with ipsilateral semitendinosus tendons, and contralateral semitendinosus tendons were used for implanting “U”-shaped on the femoral condyle to avoid the influence of the synovial fluid. Specimens were collected at 4 weeks after operation for biomechanical measurement and histological observation.
RESULTS AND CONCLUSION: Biomechanical measurement at 4 weeks after reconstruction showed that the average load of “U” shaped implanted tendon breakage was significantly higher than that of the ACL reconstructed tendon (P < 0.01). Histological observation showed that necrotic tendon tissues in bone tunnel were replaced by new bone tissues and fibrous tissues. Sharpey’s fibers and fibrocartilages were appeared on the tendon-bone interface. The number of bone cells on the tendon bone interface of the “U” shaped implanted tendon was significantly higher than that of ACL reconstructed tendon (P < 0.01), superior to intra-articular group. It results showed that biomechanics and tendon bone healing of the “U” shaped implanted tendon were both superior to the ACL reconstructed tendon. It is indicated that joint synovial fluid of ACL reconstruction has an adverse effect on ligament strength and tendon bone healing.

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