Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9633-9636.doi: 10.3969/j.issn.1673-8225.2011.51.031

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Synthesis of cholesterol-modified pullulan and its self-assembled behavior

Tao Xiao-jun1, Tang Hong-bo1, Ling Kai1, Wang Yan1, Zhang Qi-qing1, 2   

  1. 1Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin  300192, China
    2Research Center of Biomedical Engineering, Xiamen University, Xiamen  361005, Fujian Province, China
  • Received:2011-04-11 Revised:2011-09-12 Online:2011-12-17 Published:2011-12-17
  • Contact: Zhang Qi-qing, Doctor, Professor, Doctoral supervisor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China; Research Center of Biomedical Engineering, Xiamen University, Xiamen 361005, Fujian Province, China zhangqiq@126.com
  • About author:Tao Xiao-jun☆, Doctor, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Key Laboratory of Biomedical Material of Tianjin, Tianjin 300192, China taoxiaojun428@ sohu.com
  • Supported by:

    the National Major Science Research Plan of China, No. 2006CB933300*; the Doctoral Foundation, No. 20091106120051*

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Abstract:

BACKGROUND: Carboxyl group is introduced to hydrophobically modify polysaccharides, which could self-aggregate to nanoparticles with some surface charges, and it is beneficial to improving their stability and performing the good properties as a drug carrier.
OBJECTIVE: To synthesis cholesterol hydrophobically modified carboxyethyl pullulan (CHS-CEP), and to study their self-aggregated behavior in aqueous solution and characteristics of CHS-CEP nanoparticles.
METHODS: Acrylic acid and pullulan polysaccharides could form carboxyethyl pullulan (CEP) by additive reaction. Objective material could be obtained by catalyzing CEP and CSN. The synthesized material was characterized using proton magnetic resonance (1H-NMR). The nanoparticles were prepared by dialysis to characterize the morphology, size and zeta potential by transmission electron microscopy (TEM) and dynamic laser scattering (DLS).
RESULTS AND COUCLUSION: By 1H-NMR analysis, it showed that CHS-CEP was successfully synthesized and this material could self-aggregate to form nanoparticles with opalescence. The diameter of CHS-CEP nanoparticles was about 148.6 nm and their zeta potential was -19.9 mV. They could act as drug carrier due to many excellent properties such as a good dispersity in aqueous solution, even distribution and long-term stability.

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