Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9608-9612.doi: 10.3969/j.issn.1673-8225.2011.51.025

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Preparation and characterization of rifampicin-loaded poly(lactic-co-glycolic acid)  microspheres

Ye Xiang-yang1, Sun Xiang2, Jia Hui-wen3, Tang Li-xin1, Zhao Yu-guo1, Zhen Ping4   

  1. 1First Department of Orthopedics, Nanyang Central Hospital, Nanyang  473000, Henan Province, China
    2Department of Blood Transfusion, Xichuan Country People’s Hospital, Xichuan  474450, Henan Province, China
    3Emergency Department, Nanyang Central Hospital, Nanyang  473000, Henan Province, China
    4Department of Orthopedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou  730050, Gansu Province, China
  • Received:2011-03-11 Revised:2011-04-27 Online:2011-12-17 Published:2011-12-17
  • Contact: Zhen Ping, Doctor, Chief physician, Professor, Master’s supervisor, Department of Orthopedics, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu Province, China zhenpingok@163.com
  • About author:Ye Xiang-yang★, Master, Attending physician, First Department of Orthopaedics, Nanyang Central Hospital, Nanyang 473000, Henan Province，China yexyang2008@163.com

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Abstract:

BACKGROUND: Some scholars prepared rifampicin-loaded poly (lactic-co-glycolic acid) (PLGA) microspheres with diameter of 10μm which was not suitable to be combined with calcium phosphate cement to prepare repair materials with good degradation.
OBJECTIVE: To conduct the preparation of rifampicin-loaded PLGA microspheres with large size and to study the physicochemical characteristics of the microspheres and the characteristics of drug release in vitro.
METHODS: Rifampicin-loaded microspheres of biodegradable PLGA were prepared using a double-emulsion solvent-extraction [(water-in-oil)-in-water] technique. The morphology of the microspheres was observed by scanning electron microscope and light microscope. Their average diameter and the span were measured. Their envelopment rate and drug-carried rate were determined by high performance liquid chromatography. Their release characteristics were studied in vitro by dissolution method. The curve of drug release in vitro was fitted and the equation of the curve was established.
RESULTS AND CONCLUSION: Rifampicin-loaded PLGA microspheres presented complete globe, good dispersibility and no obviously aggregation. Their average diameter was (80.0±9.4) μm, drug-carried rate was (33.18±1.36)% and envelopment rate was (54.79±1.13)%. In vitro release property of the microspheres was good. In the burst release phase, the rate of drug release in vitro was (14.66±0.18)%, and rose to (18.09±0.45)% and (92.17±1.23)% 3 and 42 days later. Rifampicin-loaded PLGA microspheres have the characteristics of slowing antituberculosis drugs releasing.

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