Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (51): 9527-9530.doi: 10.3969/j.issn.1673-8225.2011.51.007

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In vitro cytotoxicity evaluation of a new type anticalcification bovine jugular venous conduit

Lin Xi1, Zhang Wei-da1, Zhang Hong-bin2, Li Jie1, Wang Xiao-li1   

  1. 1Department of Cardiovascular Surgery, 2Department of Medical Research, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou  510010, Guangdong Province, China
  • Received:2011-06-04 Revised:2011-08-07 Online:2011-12-17 Published:2011-12-17
  • Contact: Zhang Wei-da, Chief physician, Department of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China xzwkzx@163.com
  • About author:Lin Xi★, Master, Attending physician, Department of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Command of Chinese PLA, Guangzhou 510010, Guangdong Province, China linxi_military@ hotmail.com
  • Supported by:

    Science and Technology Planning Projects of Guangdong Province, No. 2007B031001008*

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Abstract:

BACKGROUND: Triton X-100 and epoxy chloropropane modification with glutaraldehyde-treated bovine jugular venous conduit is a new type anticalcification valved conduit for right ventricular outflow tract reconstruction. There are few reports on the biocompatibility research.
OBJECTIVE: To evaluate the cytotoxicity of the new type anticalcification bovine jugular venous conduit in vitro.
METHODS: The cytotoxicity of the new type anticalcification bovine jugular venous conduit (experimental group) and only glutaraldehyde treated bovine jugular venous conduit extracts (control group) was detected by CCK-8. The relative growth rate was calculated and the toxicity was graded at days 2 and 4.
RESULTS AND CONCLUSION: CCK-8 test results showed that the relative growth rate of the experimental group was more than 85%. The toxicity grade was 1, and there was non-cytotoxicity, which were significantly better than those in the control group (P < 0.05). It was indicated that Triton X-100 and epoxy chloropropane modification with glutaraldehyde-treated bovine jugular venous conduit had no cytotoxicity and should be a safe implanted material.

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