Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9425-9427.doi: 10.3969/j.issn.1673-8225.2011.50.028

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Construction of a eukaryotic expression vector containing mouse heat shock transcription factor 1

Ouyang Hua-wei1, Tan Jun1, Li Gao-feng1, Luo Cheng-qun2   

  1. 1Department of Plastic and Laser Cosmetic Surgery, People’s Hospital of Hunan Province, Changsha  410005, Hunan Province, China
    2Department of Burn and Plastic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China
  • Received:2011-10-17 Revised:2011-11-07 Online:2011-12-10 Published:2011-12-10
  • Contact: Luo Cheng-qun, Professor, Doctoral supervisor, Chief physician, Department of Burn and Plastic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, Hunan Province, China LCQ6666@163.com
  • About author:Ouyang Hua-wei☆, Doctor, Physician, Department of Plastic and Laser Cosmetic Surgery, People’s Hospital of Hunan Province, Changsha 410005, Hunan Province, China benchi2008165@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30672035*

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Abstract:

BACKGROUND: Heat shock transcription factor 1 has heat shock response effect.
OBIECTIVE: To construct a eukaryotic expression vector containing mouse heat shock transcription factor 1 (HSF1).
METHODS: Amplified mouse HSF1 cDNA fragment was cloned into pcDNA3.1 to construct a eukaryotic expression vector pcDNA3.1-HSF1.
RESULTS AND CONCLUSION: There were two fragments in electrophoresis after restriction enzyme digestion, 5.5 kb fragment and 1.5 kb fragment. The result of DNA sequencing showed that the sequence of the cloned HSF1 was identical to that GeneBank had reported. HSF1 protein band with the relative molecular mass of 72 000 was detected by western blot. These findings indicate that the eukaryotic expression vector pcDNA3.1-HSF1 containing mouse HSF1 is successfully constructed.

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