Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9415-9418.doi: 10.3969/j.issn.1673-8225.2011.50.026

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Effect of long needle penetration needling on matrix metalloproteinase 3 in the synovium of knee osteoarthritis model rats

Li Shi-sheng1, 2, Wu Yao-chi2, Zhang Jun-feng2, Zhou Jing-hui3, Sun Yi-jun2, Huang Cheng-fei2   

  1. 1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai Residency Standardized Training Base, Shanghai  200233, China; 2Sixth People’s Hospital of Shanghai, Shanghai  200233, China
  • Received:2011-06-01 Revised:2011-07-13 Online:2011-12-10 Published:2011-12-10
  • Contact: Wu Yao-chi, Professor, Chief physician, Doctoral supervisor, Sixth People’s Hospital of Shanghai, Shanghai 200233, China wuyaochi@online.sh.cn
  • About author:Li Shi-sheng★, Master, Physician, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai Residency Standardized Training Base, Shanghai 200233, China lishishengshitou@126.com
  • Supported by:

    the Scientific Research Program of Shanghai Science and Technology Committee, No. 08DZ1973200*; the Scientific Research Foundation for Traditional Chinese Medicine of Shanghai Health Bureau, No. 2008L010A*

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Abstract:

BACKGROUND: Studies have shown that matrix metalloproteinases 3 plays an important role in articular cartilage degeneration and destruction.
OBJECTIVE: To observe the changes of metalloproteinases 3 expression level in synovium of knee osteoarthritis rats after treated with penetration needling of long needle and sodium hyaluronate.
METHODS: SD rats were injected with 1.6% papain solution to knee joint cavity and were driven to exercise in order to construct knee osteoarthritis model. Rats were randomly assigned into 3 groups in the 2nd week after model construction: penetration needling group, drug group and model group. Penetration needling group were treated by penetration needling with filiform needle, with a diameter of 0.30 millimeters and a length of 125 milimeters. Drug group were injected with sodium hyaluronate to knee joint cavity. Model group received no treatment. And non-model control group were established.
RESULTS AND CONCLUSION: Content of synovium matrix metalloproteinase 3 in model group was significantly increased than that in the control group (P < 0.05) in the 4th week after treatment. The content of matrix metalloproteinase 3 in penetration needling group and drug group was significantly reduced than that in the model group (P < 0.05). Penetration needling group was similar to drug group (P > 0.05). These findings demonstrate that long needle penetration therapy can effectively correct the abnormal expression of matrix metalloproteinase 3 in synovium of knee osteoarthritis model rats. It may be one of the mechanisms of the treatment of knee osteoarthritis by long needle penetration needling.

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