Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (50): 9389-9392.doi: 10.3969/j.issn.1673-8225.2011.50.020

Previous Articles     Next Articles

Gene expression of myelin associated protein in adult rats after focal cerebral ischemia and reperfusion

Li Hua-jie1, Wu Jian1, Zhu Lin-feng1, Bao Shi-yao2   

  1. 1Department of Neurology, Third Affiliated Hospital of Soochow University (First People’s Hospital of Changzhou), Changzhou  213003, Jiangsu Province, China
    2Department of Neurology, Second Affiliated Hospital of Soochow University, Suzhou  215004, Jiangsu Province, China
  • Received:2011-06-18 Revised:2011-08-27 Online:2011-12-10 Published:2011-12-10
  • Contact: Li Hua-jie, Department of Neurology, Third Affiliated Hospital of Soochow University (First People’s Hospital of Changzhou), Changzhou 213003, Jiangsu Province, China lihuajie0589@yahoo.com.cn
  • About author:Li Hua-jie☆, Doctor, Associate chief physician, Department of Neurology, Third Affiliated Hospital of Soochow University (First People’s Hospital of Changzhou), Changzhou 213003, Jiangsu Province, China lihuajie0589@yahoo.com.cn
  • Supported by:

    the Changzhou Foundation for Social Development, No. cs2005206*

PDF

364

Abstract:

BACKGROUND: Myelin proteins are the main components in oligodendroglia cells. Research on gene expression changes of myelin related proteins after focal cerebral ischemia and reperfusion will help to understand the role of oligodendroglia cells in focal cerebral ischemia and reperfusion.
OBJECTIVE: To explore the gene expression change of myelin related proteins in different cerebral regions and at different time points after focal cerebral ischemia and reperfusion in adult model rats.
METHODS: Focal cerebral ischemia and reperfusion model in adult SD rats was constructed using suture method. The early phase mRNA expression changes of myelin proteolipid protein, myelin basic protein and myelin transcription factor 1 in the cerebral cortex regions of infraction center, infarction borders and contralateral area of infarction were detected by fluorescent in situ hybridization with the dig-labeled oligonucleotides.
RESULTS AND CONCLUSION: The gene expression of the three myelin related proteins in infraction center decreased significantly in the early phase of focal cerebral ischemia and reperfusion. In infarction borders, there was no significant change in the gene expression of the three myelin related proteins between experiment group and control group on the 1st day after focal cerebral ischemia and reperfusion. Then the gene expression of experiment group increased, and exceeded that of the control group on the 14th day after focal cerebral ischemia and reperfusion (P < 0.05, 0.01); the content of myelin transcription factor 1 positive cells raised the earliest (P < 0.01). The gene expression of cerebral cortex myelin related proteins in infarction borders increased after focal cerebral ischemia and reperfusion in adult SD rats. It indicates that oligodendrocyte precursors are sensitive to cerebral ischemic injury and may involve in the repairing process of cerebral post-ischemic injury.

CLC Number: