Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8681-8684.doi: 10.3969/j.issn.1673-8225.2011.46.031

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Chymase expression in deep partial-thickness burn wounds after burn injury in hamsters

Dong Xiang-lin1, Geng Zhong-li2, Gao Wei-cheng1, Zhao Yang1, Qin Tao1, Qiao Xing1, Ma Juan1, Ma Shao-lin1   

  1. 1Department of Burns and Plastic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
    2Department of Breast, Head and Neck Surgical Oncology, Affiliated Tumour Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uygur Autonomous Region, China
  • Received:2011-04-19 Revised:2011-08-16 Online:2011-11-12 Published:2011-11-12
  • Contact: Ma Shao-lin, Master, Chief physician, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China mashaolin9@163.com
  • About author:Dong Xiang-lin★, Master, Associate chief physician, Department of Burns and Plastic Surgery, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    Youth Science Foundation of the First Affiliated Hospital of Xinjiang Medical University, No. 2010QN11*

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Abstract:

BACKGROUND: Little is known about mast cell chymase in burn injury in hamsters.
OBJECTIVE: To investigate whether mast cell chymase is involved in burn injury and the change of chymase expression in burn wound.
METHODS: Deep partial-thickness scald in hamsters was induced with contact time of 12 seconds by 20 mL 75 ℃ water in a   50 mL syringe. Chymase mRNA level and chymase activity were analyzed before burn and at 1, 3, 7, 14 days after burn in deep second-degree scald wound.
RESULTS AND CONCLUSION: Quantitative real-time RT-PCR and radioimmunoassay results showed that chymase gene expression and chymase activity in burn wound were significantly increased after burn and were highest at 3 days after burn. Expression of mast cell chymase would be involved in burn injury and changed at different stages after burn in hamsters.

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