Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (46): 8653-8656.doi: 10.3969/j.issn.1673-8225.2011.46.024

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Olfactory ensheathing cell viability in different cryopreservation systems

Liu Yu-liang1, Wei Kai-bin1, Liu Hong2, He Yu-qin2, Zhuo Feng1 , Lü Xin-gang3   

  1. 1First Department of Orthopedics, 2Nursing Department, Taian Central Hospital, Taian  271000, Shandong Province, China; 3First Department of Orthopedics, Xinwen Coal Mining Bureau Laiwu Central Hospital, Laiwu  271100, Shandong Province, China
  • Received:2011-01-19 Revised:2011-05-18 Online:2011-11-12 Published:2011-11-12
  • Contact: Wei Kai-bin, Doctor, Chief physician, First Department of Orthopedics, Taian Central Hospital, Taian 271000, Shandong Province, China wkb1966@163.com
  • About author:Liu Yu-liang★, Master, Attending physician, First Department of Orthopedics, Taian Central Hospital, Taian 271000, Shandong Province, China yuliang-liu@163.com
  • Supported by:

    the Science and Technology Development Plan Project of the Medicine and Health of Shandong Province, No. 2009HW091*

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Abstract:

BACKGROUND: A proper preservation method would be of important significance for experiments and clinical application of olfactory ensheathing cells (OECs)
OBJECTIVE: To explore proper cyropreservative systems for OECs.
METHODS: OECs during the logarithmic growth phase were harvested, cryopreserved for 1, 3 and 6 months and then revitalized.
RESULTS AND CONCLUSION: MTT assay and tryplan blue staining showed that cells exhibited highest viability after treatment with 5% dimethyl sulfoxide (DMSO)-6% hydroxyethyl starch (HES), followed by 10% DMSO, and lastly the 5% DMSO. Use of refrigerator or cryogenic control system with different cryopreservation time did not yield obvious effects on viability of OECs. Therefore, 5% DMSO-6%HES is recommended as a cryopreservative agent for OECs.

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